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リコンビナントRabMAb

Anti-HSF1 (phospho S326) 抗体 [EP1713Y] (ab76076)

Reviews (1)Q&A (1)References (38)
Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HSF1 (phospho S326) antibody [EP1713Y]
    HSF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cell lysates and HeLa cell lysate treated with heat (44ºC)

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76076の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
Use a concentration of 0.338 µg/ml.
WB (1)
1/5000 - 1/10000. Predicted molecular weight: 57 kDa.
特記事項
Dot blot
Use a concentration of 0.338 µg/ml.
WB
1/5000 - 1/10000. Predicted molecular weight: 57 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

ターゲット情報

  • 機能

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

  • 配列類似性

    Belongs to the HSF family.

  • ドメイン

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

  • 翻訳後修飾

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

  • 細胞内局在

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    画像

    • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 0.169 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa treated with heat at 43? for 30 minutes whole cell lysate
      Lane 3 : HeLa treated with heat at 43? for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 µg/ml

      Predicted band size: 57 kDa
      Observed band size: 82 kDa why is the actual band size different from the predicted?



      Blocking/Diluting Buffer and concentration: 5% NFDM /TBST

    • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 1/10000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates. Then the membrane was incubated with phosphatase

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 82 kDa why is the actual band size different from the predicted?

    • Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

      Dot blot analysis of HSF1 (phospho S326) peptide (Lane 1), HSF1 non-phospho peptide (Lane 2) labelling HSF1 (phospho S326) with purified ab76076 at 0.338 ug/mL. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 ug/mL.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

    参考文献 (38)

    ab76076 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76076 は 38 報の論文で使用されています。

    • Lee S  et al. Targeting HSF1 as a Therapeutic Strategy for Multiple Mechanisms of EGFR Inhibitor Resistance in EGFR Mutant Non-Small-Cell Lung Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34203709
    • Inda MC  et al. The epichaperome is a mediator of toxic hippocampal stress and leads to protein connectivity-based dysfunction. Nat Commun 11:319 (2020). PubMed: 31949159
    • Paszek A  et al. Heat shock response regulates stimulus-specificity and sensitivity of the pro-inflammatory NF-?B signalling. Cell Commun Signal 18:77 (2020). PubMed: 32448393
    • Mun GI  et al. Decreased expression of FBXW7 by ERK1/2 activation in drug-resistant cancer cells confers transcriptional activation of MDR1 by suppression of ubiquitin degradation of HSF1. Cell Death Dis 11:395 (2020). PubMed: 32457290
    • Zhou G  et al. Role of the Receptor for Advanced Glycation End Products in Heat Stress-Induced Endothelial Hyperpermeability in Acute Lung Injury. Front Physiol 11:1087 (2020). PubMed: 33192536
    View all Publications for this product

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-HSF1 (phospho S326) antibody [EP1713Y]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon)
    Loading amount
    20 µg
    Specification
    colon
    Treatment
    heat shock
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Young Ju Yoon

    Verified customer

    投稿 Sep 04 2009

    Question

    Inquiry: this antibody, as written on the datasheet, does not react with mouse tissue and murine species.... ONLY human (also on epitomics website) Lindquist (Cell and JCI...) has published the usage of this ab in mice cell line and tissues..... please could you confirm that ab76076 and ab81281 work on WB on mice cells and mice tissues, thank you best regards

    Read More
    Answer

    Thank you for contacting us and bringing this to our attention.


    We have tested both products (ab76076 and ab81281) in mouse and rat samples, via WB.
    The samples we tested were brain, heart, kidney and spleen for each, in mouse and rat. We only saw a few non-specific bands (with both products) in mouse kidney. No bands were observed in the other lysates.
    We do not recommend these products for use in mouse and rat based on this testing.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com