製品の概要
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製品名Anti-HSD17B4 antibody
HSD17B4 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HSD17B4
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由来種Rabbit
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アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
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種交差性交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Pig
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免疫原
Recombinant fragment corresponding to Human HSD17B4 aa 355-615.
Database link: NP_000405 -
ポジティブ･コントロール
- HepG2 whole cell lysate; DLD-1 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab97975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
ターゲット情報
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機能Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
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組織特異性Present in many tissues with highest concentrations in liver, heart, prostate and testis.
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パスウェイLipid metabolism; fatty acid beta-oxidation.
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関連疾患D-bifunctional protein deficiency
Perrault syndrome 1 -
配列類似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 MaoC-like domain.
Contains 1 SCP2 domain. -
細胞内局在Peroxisome.
- Information by UniProt
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参照データベース
- Entrez Gene: 3295 Human
- Entrez Gene: 15488 Mouse
- Entrez Gene: 79244 Rat
- Omim: 601860 Human
- SwissProt: P51659 Human
- SwissProt: P51660 Mouse
- SwissProt: P97852 Rat
- Unigene: 406861 Human
see all -
別名
- 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
- 17 beta HSD 4 antibody
- 17 beta HSD IV antibody
see all
画像
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Western blot - Anti-HSD17B4 antibody (ab97975)Anti-HSD17B4 antibody (ab97975) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)Immunohistochemical analysis of HSD17B4 in paraffin-embedded DLD-1 xenograft, using ab97975 at 1/500 dilution.
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Western blot - Anti-HSD17B4 antibody (ab97975)Anti-HSD17B4 antibody (ab97975) at 1/3000 dilution + Rat heart lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 80 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)ab97975 staining HSD17B4 in rat heart tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)ab97975 staining HSD17B4 in rat testis tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-HSD17B4 antibody (ab97975)ab97975 staining HSD17B4 in methanol- fixed Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence) at a 1:500 dilution.
参考文献
ab97975 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"