Anti-HSD17B14 抗体 (ab272589)
Key features and details
- Rabbit polyclonal to HSD17B14
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HSD17B14 antibody
HSD17B14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HSD17B14 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human HSD17B14 aa 1-100.
Database link: Q9BPX1 -
ポジティブ・コントロール
- WB: HEK-293T. IHC-P: Human stomach tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272589の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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関連性
HSD17B14 belongs to the short-chain dehydrogenases/reductases (SDR) family. It has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity and converts oestradiol to oestrone. The physiological substrate is not known. HSD17B14 acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro). It is highly expressed in brain, placenta, liver and kidney. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 51171 Human
- Omim: 612832 Human
- SwissProt: Q9BPX1 Human
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別名
- 17 beta HSD 14 antibody
- 17 beta hydroxysteroid dehydrogenase 14 antibody
- 17 beta hydroxysteroid dehydrogenase DHRS10 antibody
see all
画像
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Lanes 2-3 : Anti-HSD17B14 antibody (ab272589) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Control (vector only transfected HEK-293T lysate)
Lane 3 : HSD17B14 over-expression lysate (mammalian HEK-293T cells) -
Paraffin-embedded formalin-fixed Human stomach tissue stained for HSD17B14 using ab272589 at 1/50 in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272589 は論文での使用が確認できていません。