Anti-HSD11B1 抗体 (ab39364)
Key features and details
- Rabbit polyclonal to HSD11B1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HSD11B1 antibody
HSD11B1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HSD11B1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Hamster, Cow, Cat, Dog, Monkey, Cynomolgus monkey, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human HSD11B1 aa 50-150.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab39364 is an affinity purified IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39364の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). |
ターゲット情報
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機能
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. -
組織特異性
Widely expressed. Highest expression in liver. -
関連疾患
Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). -
配列類似性
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
翻訳後修飾
Glycosylated. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3290 Human
- Omim: 600713 Human
- SwissProt: Q6R0J2 Hamster
- SwissProt: P28845 Human
- SwissProt: Q7M3I4 Rabbit
- Unigene: 195040 Human
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別名
- 11 beta HSD 1 antibody
- 11 beta HSD1 antibody
- 11 beta hydroxysteroid dehydrogenase 1 antibody
see all
画像
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Western blot analysis of Sheep liver tissue lysate (5µg/lane) labeling HSD11B1 with ab39364 at 1/500 (16 hours at 4ºC). An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Anti-HSD11B1 antibody (ab39364) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Exposure time: 16 minutes
HSD11B1 contains a number of potential glycosylation sites (SwissProt) which may explain the higher migration pattern observed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab39364 は 25 報の論文で使用されています。
- Leinroth AP et al. Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations. Cell Rep 39:110785 (2022). PubMed: 35545045
- Guan H et al. 5-Bis-(2,6-difluoro-benzylidene) Cyclopentanone Acts as a Selective 11ß-Hydroxysteroid Dehydrogenase one Inhibitor to Treat Diet-Induced Nonalcoholic Fatty Liver Disease in Mice. Front Pharmacol 12:594437 (2021). PubMed: 33912032
- Miljanovic N et al. Proteomic signature of the Dravet syndrome in the genetic Scn1a-A1783V mouse model. Neurobiol Dis 157:105423 (2021). PubMed: 34144125
- Ren J et al. Impact of maternal late gestation undernutrition on surfactant maturation, pulmonary blood flow and oxygen delivery measured by magnetic resonance imaging in the sheep fetus. J Physiol 599:4705-4724 (2021). PubMed: 34487347
- Elakovic I et al. Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats. Nutrients 12:N/A (2020). PubMed: 33198224