Anti-Hsc70 抗体 (ab1427)
製品の概要
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製品名
Anti-Hsc70 antibody
Hsc70 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsc70 -
由来種
Rabbit -
特異性
The antibody detects a 73 kDa protein, corresponding to the apparent molecular weight of Hsc70 on SDS-PAGE immunoblots. No reactivity with inducible Hsp70 is detected. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Hamster, Cow, Human, Pig, Monkey -
免疫原
Synthetic peptide:
EKLQGKINDEDKQKI
, corresponding to amino acids 555-570 of Human Hsc70 -
特記事項
The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol, 0.5% BSA, 0.015% EDTA -
Concentration information loading... -
一次抗体 備考
The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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機能
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the heat shock protein 70 family. -
ドメイン
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
細胞内局在
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 281831 Cow
- Entrez Gene: 3312 Human
- Entrez Gene: 15481 Mouse
- Entrez Gene: 24468 Rat
- Omim: 600816 Human
- SwissProt: P11142 Human
- SwissProt: P63017 Mouse
- SwissProt: P63018 Rat
see all -
別名
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab1427)Ab1427 staining human normal testis tissue. Staining is localised to cytoplasmic and nuclear compartments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplif -
Western blot - Anti-Hsc70 antibody (ab1427)This image is courtesy of an anonymous Abreview.All lanes : Anti-Hsc70 antibody (ab1427) at 1/2500 dilution
Lane 1 : U2OS cell lysate
Lane 2 : HeLa cell lysate
Secondary
All lanes : HRP-conjugated Goat polyclonal to Rabbit at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 2 minutes
プロトコール
参考文献
ab1427 は 11 報の論文で使用されています。
- Cao Y et al. Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant. Nat Commun 9:3695 (2018). PubMed: 30209303
- Xu CY et al. DJ-1 Inhibits a-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy. Front Aging Neurosci 9:308 (2017). PubMed: 29021755
- Soleilhavoup C et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). PubMed: 26518761
- Gao L et al. Oxidation of survival factor MEF2D in neuronal death and Parkinson's disease. Antioxid Redox Signal 20:2936-48 (2014). PubMed: 24219011
- Dermentzaki G et al. Loss of ß-glucocerebrosidase activity does not affect alpha-synuclein levels or lysosomal function in neuronal cells. PLoS One 8:e60674 (2013). WB ; Human . PubMed: 23580063
