HRP Anti-UQCRFS1/RISP 抗体 [5A5] (ab198392)
Key features and details
- HRP Mouse monoclonal [5A5] to UQCRFS1/RISP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-UQCRFS1/RISP antibody [5A5]
UQCRFS1/RISP 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [5A5] to UQCRFS1/RISP -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human heart mitochondria lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
5A5 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab198392の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
配列類似性
Contains 1 Rieske domain. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
- Unigene: 726119 Human
see all -
別名
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
画像
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HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198392 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab198392 は論文での使用が確認できていません。