HRP Anti-Rabbit IgG 抗体 [SP137] (ab270144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [SP137] to Rabbit IgG
- Suitable for: IHC-P
- Reacts with: Rabbit
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-Rabbit IgG antibody [SP137]
Rabbit IgG 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [SP137] to Rabbit IgG -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rabbit -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.88% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
SP137 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270144の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
細胞内局在
Secreted -
参照データベース
- SwissProt: P01838 Rabbit
- SwissProt: P01839 Rabbit
- SwissProt: P01840 Rabbit
- SwissProt: P01841 Rabbit
- SwissProt: P01847 Rabbit
- SwissProt: P01870 Rabbit
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別名
- Ig gamma chain C region antibody
- Ig kappa-b4 chain C region antibody
- Ig kappa-b5 chain C region antibody
see all
画像
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IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND®.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal mouse spleen*, performed on a Leica BOND®.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal rat spleen*, performed on a Leica BOND®.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab270144 は 8 報の論文で使用されています。
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- Chang H & Yao Y lncRNA TMPO antisense RNA 1 promotes the malignancy of cholangiocarcinoma cells by regulating let-7g-5p/ high-mobility group A1 axis. Bioengineered 13:2889-2901 (2022). PubMed: 35040749
- Li H et al. Chelerythrine Chloride Inhibits Stemness of Melanoma Cancer Stem-Like Cells (CSCs) Potentially via Inducing Reactive Oxygen Species and Causing Mitochondria Dysfunction. Comput Math Methods Med 2022:4000733 (2022). PubMed: 35761835
- Cai MY et al. Role of Carbon Monoxide in Oxidative Stress-Induced Senescence in Human Bronchial Epithelium. Oxid Med Cell Longev 2022:5199572 (2022). PubMed: 36193088
- Zhao S et al. PD-L1 Regulates Inflammation in LPS-Induced Lung Epithelial Cells and Vascular Endothelial Cells by Interacting with the HIF-1a Signaling Pathway. Inflammation N/A:N/A (2021). PubMed: 34014492
- Lu X et al. MicroRNA-186-3p attenuates tumorigenesis of cervical cancer by targeting MCM2. Oncol Lett 22:539 (2021). PubMed: 34084218
- Wu K et al. MicroRNA-18a-5p regulates the Warburg effect by targeting hypoxia-inducible factor 1α in the K562/ADM cell line. Exp Ther Med 22:1069 (2021). PubMed: 34447462
- Sun J et al. Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma. Bioengineered 12:9753-9765 (2021). PubMed: 34696681