アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    hrp-protein-a-antibody-ab18596.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Fusion / Marker Proteins Protein A
Share by email

HRP Anti-Protein A 抗体 (ab18596)

  • Datasheet
  • SDS
Submit a review Q&A (12)References (1)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

ELISA - Anti-Protein A antibody (HRP) (ab18596)

    Key features and details

    • HRP Chicken polyclonal to Protein A
    • Suitable for: ELISA
    • Reacts with: Staphylococcus aureus
    • Conjugation: HRP
    • Isotype: IgY

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-ARID1A antibody [EPR13501] (ab182560)
    一次抗体
    Product image
    Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463)
    IHC
    Product image
    Biotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use) (ab64256)

    関連製品

    医薬用外劇物

    製品の概要

    • 製品名

      HRP Anti-Protein A antibody
      Protein A 一次抗体 製品一覧
    • 製品の詳細

      HRP Chicken polyclonal to Protein A
    • 由来種

      Chicken
    • 標識

      HRP
    • アプリケーション

      適用あり: ELISAmore details
    • 種交差性

      交差種: Staphylococcus aureus
    • 免疫原

      Recombinant full length protein corresponding to Protein A.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C.
    • バッファー

      pH: 7.00
      Preservative: 0.002% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      ab18596 was immunoaffinity purified off protein A agarose and conjugated with horseradish peroxidase (HRP).
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgY
    • 研究分野

      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • Protein A

    関連製品

    • Recombinant Protein

      • Recombinant Staphylococcus aureus Protein A (ab52953)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab18596の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    1/5000 - 1/20000.
    特記事項
    ELISA
    1/5000 - 1/20000.

    ターゲット情報

    • 関連性

      Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
    • 細胞内局在

      Secreted; cell wall.
    • 参照データベース

      • Entrez Gene: 3919448 Staphylococcus aureus
      • SwissProt: B3GGB2 Staphylococcus aureus
      • SwissProt: P02976 Staphylococcus aureus
      • SwissProt: P38507 Staphylococcus aureus
      • 別名

        • gG-binding protein A antibody
        • Immunoglobulin G binding protein A antibody
        • SPA antibody
        • Staphylococcal protein A antibody

      画像

      • ELISA - Anti-Protein A antibody (HRP) (ab18596)
        ELISA - Anti-Protein A antibody (HRP) (ab18596)

      プロトコール

      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (1)

      ab18596 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab18596 は 1 報の論文で使用されています。

      • Dong L  et al. A Positive Feedback Loop of lncRNA DSCR8/miR-98-5p/STAT3/HIF-1a Plays a Role in the Progression of Ovarian Cancer. Front Oncol 10:1713 (2020). PubMed: 32984052

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 12 Abreviews or Q&A

      Question

      Dear Madam or Sir, in a previous request I asked for the availability of an antibody and got the answer below. "For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials of different stocks of this antibody in the UK.' I am surprised to hear that there are only 9 vials of that product in stock. Does that mean there is a problem with resupply? Do you plan on generating more lots?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 02 2012

      Answer

      Thank you for contacting us.

      Worldwide, we have 16 vials of this antibody in stock. We do not have more as it is not a bestseller, but sells quite solid. This product is not a niche product either, therefore, we constantly have have a certain threshold in stock.

      In case you want to buy a higher quantity of a certain lot, please let our customer service team, so we can order it with the lab for you.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Nov 02 2012

      Question

      Did you also evaluate the signal of protein A bound to antibodies (if so, did you denature, and how)? Did you compare the specific signal to a blank signal?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 07 2013

      Answer

      It appears that you are asking if a capture(sandwich) ELISA was performed.

      We do not run a capture test on the the conjugate, only a direct ELISA to determine the functionality of the conjugation.

      Read More

      Abcam Scientific Support

      Answered on Jun 07 2013

      Question

      Could you please send me expiry dating and data on the following products/lots? Chicken polyclonal antibody to Protein A (1 mg/mL from Abcam Cat. # ab19483) Lot: GR58389-4 Chicken polyclonal antibody to Protein A (HRP) (1 mg/mL from Abcam Cat. # ab18596) Lot: GR105326-2

      Read More

      Abcam community

      Verified customer

      Asked on Apr 17 2013

      Answer

      We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols). However, we do suggest the following approximate use by dates for all products based from timne of delivery: room temperature: 1 week + 4°C: 1 year - 20°C: 5 years - 80°C: 10 years These products are covered by our Abpromise guarantee fro a period of one year. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within twelve months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.

      Read More

      Abcam Scientific Support

      Answered on Apr 17 2013

      Question

      Dear Madam or Sir, we would be interested in knowing how many vials of the product no 19483, lots GR58389 - X and prod no 18596 lots GR74278 - X are still available. Is it correct that the last number of the lot (indicated here as X) indicates the filling date and that the batches filled on different days should be comparable? I am looking forward to you reply.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 02 2012

      Answer

      Thank you for contacting us.

      Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

      For ab19483, chicken polyclonal to Protein A, we have 16 vials of lot GR58389-4 in our US office ready to ship and an additional 30 units of lots GR58389-3 and GR58389-4 in our UK office.

      For ab18596, chicken polyclonal to Protein A (HRP), we have 1 vial of GR74278-4 in our UK office which would take a few extra days to ship to you. We have two new lots in for that antibody, but only 1 vial left of the lot you requested.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Nov 02 2012

      Question

      Inquiry: Dear Madam or Sir, we would be interested in knowing how many vials of the product no 19483, lots GR58389 - X and prod no 18596 lots GR74278 - X are still available. Is it correct that the last number of the lot (indicated here as X) indicates the filling date and that the batches filled on different days should be comparable? I am looking forward to you reply.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 02 2012

      Answer

      Thank you for contacting us.

      We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

      For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials of different stocks of this antibody in the UK.

      The way we number our lots is indeed the way you mentioned. The first part of the lot number e.g GR12345 corresponds to one particular lot. The last number e.g GR12345-X corresponds to the a particular date where we receieved the antibody from our labs. This means that lots GR12345-1 and GR12345-2 of a particularly antibody originate from the same stock but were delivered to our stock rooms at different days therefore these should be identical.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Nov 02 2012

      Question

      Inquiry: I want to know if protA EIAs from a specific lot number are still available, Chk pAb anti-Protein A, ab19483, GR58389-2 AND Chk pAb anti-Protein A - HRP, ab18596, GR74278-2.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 30 2012

      Answer

      Thank you for your enquiry and your interest in our products.

      I have checked the stock viability and can confirm the followings:

      - ab19483 - several vials from the same bleed and purification;

      - ab18596 - only one vial left from the batch required

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Oct 30 2012

      Question

      Currently we are working with two of your antibodies: Chicken polyclonal to Protein A (ab19483) and Anti-Protein A antibody-HRP (ab18596). On your product sheet I found that the Chicken polyclonal to Protein A is IgY an the Anti-Protein A antibody-HRP is IgG. However, both anti-bodies are derived from chicken, so I was thinking they should both be IgY?

      Looking on the internet I found that chicken IgG and chicken IgY are two different names for the same antibody, which are used interchangeably. However, for me it was a bit confusing. Therefore, I was wondering whether you can confirm that both these anti-bodies (ab19483 and ab18596) are the same isotype?

      Are they actually the same anti-body with and without HRP-conjugate or is there a reason why one of them is called IgG and the other IgY?

      Thanks in advance,

      Read More

      Abcam community

      Verified customer

      Asked on Jul 19 2012

      Answer

      Thank you for contacting us. I apologize for being late in getting back to you.

      I can confirm that both antibodies ab18596 and ab19483 have similar IgY isotype.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Jul 19 2012

      Question

      I confirmed that order number is ###### of ab18596(lot.GR27429-2) and he purchased 33 vials of this antibody so far.
      He understood about guarantee duration but I think he knows well this antibody and if there's some problem, he needs to get compensate for it.
      Please let me know if credit note is available in this case.
      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Jun 01 2012

      Answer

      Thank you for confirming this information and for your help and cooperation with this case.

      As a special exception as the customer has used so many vials succesfully, we can extend the guarantee period in this case and Iasked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

      Credit ID: 21177

      As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

      I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

      Read More

      Abcam Scientific Support

      Answered on Jun 01 2012

      Question

      Our customer obtained different result from ab18596 despite in the same conditions so,

      Please arrange a credit note.

      And he would like to receive CoA of two vials


      Ab18596-1/ lot# 793467

      Ab18596/lot# GR27429-2


      Ab18596 and ab18569-1 are the same antibody, right?

      Because of the slight difference, our customer would like to know if they are exactly the same antibodies and reason for change.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 21 2012

      Answer

      Thank you for your reply.

      I appreciate your cooperation and understand your concerns. It is regrettable the results from lot 793467have not been assatisfactory for the customer. I can confirm that ab18596 and ab18596-1 are the same antibody. The -1 just relates to the size.

      (I assume there was a typing error in your email, ab18596 and ab18569-1 are not the same antibody).

      With regards to the COA request, some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products. As the identity of our collaborators is confidential, we provide a certificate of compliance (COC)for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product. I am going to request the QC testing data for these 2 lots from our source, I assume this is what your customer would like to see for their reassurance?

      In order to help with our investigation of this case before deciding how to proceed, I would appreciate if you are able to provide more details. This information will also be helpful to our quality monitoring.

      1. Please provide the order numbers and date of purchase.

      2. I would appreciate if you are able to provide the data from each of these lots in order to help us assess the results.

      Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

      Read More

      Abcam Scientific Support

      Answered on May 21 2012

      Question

      Our customer purchased ab18596-1(lot.793467) and ab18596(lot.GR27429-2).

      He would like to know if they have differences (I have already explained about lot# difference) because when he tested lot.793467, detected OD value after 10 min incubation of TMB but, lot.GR27429-2, he observed color change within 5 min. He applied the same concentration so he wants to confirm this.

      Read More

      Abcam community

      Verified customer

      Asked on May 18 2012

      Answer

      As this is a polyclonal antibody, some batch variability could be expected. It is possible there could also be variabilitywith the detection kit or secondary antibodyin this case.

      Could you confirm if the customer is satisfied with the results with the longer incubation? We would be pleased to investigate this further if the results are not as the customer is expecting.

      I would like to reassure you that this antibody is tested and covered by our Abpromise guarantee for ELISA. In the event that a product is not functioning in the tested applications and species cited on the product data sheet, we will be pleased to provide a credit note, free of charge replacement or refund.

      www.abcam.com/abpromise

      Read More

      Abcam Scientific Support

      Answered on May 18 2012

      1-10 of 12 Abreviews or Q&A

      •  戻る
      • 1
      • 2
      • 次へ 

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      技術情報や製品情報をメールで受け取る 登録
      製品・研究分野別
      • 癌
      • 循環器
      • 細胞生物学
      • 発生
      • エピジェネティクス&核内情報
      • 免疫学
      • メタボリズム
      • 感染症
      • ニューロサイエンス
      • シグナル伝達
      • ステムセル
      製品・タイプ別
      • 一次抗体
      • 二次抗体
      • バイオケミカルズ
      • アイソタイプ・コントロール
      • マルチカラー・セレクター
      • キット
      • ローディング・コントロール
      • ライゼート
      • ペプチド
      • タンパク質
      • スライド
      • タグ抗体・細胞マーカー
      • その他試薬
      サービス&サポート
      • サポート
      • よくあるお問い合わせ
      • プロトコール&トラブルシュート
      • ご購入に関して
      • お問い合わせフォーム
      • トレーニング
      • ターゲット別に見る
      アブカムについて
      • 企業ウェブサイト
      • 投資家の皆様へ
      • 企業情報
      • 採用情報
      • アブカムについて
      イベント
      • 展示会
      • カンファレンス
      International websites
      • abcam.com
      • abcam.cn

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      © 1998-2022 Abcam plc. All rights reserved.