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HRP Anti-proBNP 抗体 [24E11] (ab13123)

Submit a review Q&A (1)References (1)
Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)

    Key features and details

    • HRP Mouse monoclonal [24E11] to proBNP
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Conjugation: HRP
    • Isotype: IgG

    Conjugates logo Related conjugates and formulations

    Unconjugated

    製品の概要

    • 製品名

      HRP Anti-proBNP antibody [24E11]
      proBNP 一次抗体 製品一覧

    • 製品の詳細

      HRP Mouse monoclonal [24E11] to proBNP

    • 由来種

      Mouse

    • 標識

      HRP

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Recombinant fragment

    • 免疫原

      Synthetic peptide:

      RGHRKMVLYTLRAPR

      , corresponding to amino acids 61 - 76 of Human proBNP.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.40
      Preservative: 0.05% Proclin 300
      Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
    • Concentration information loading...

    • 精製度

      Protein A purified

    • 特記事項（精製）

      Chromatography on protein A Sepharose Purified from TCS

    • ポリ/モノ

      モノクローナル

    • クローン名

      24E11

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab13123の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
    特記事項
    WB
    Use at an assay dependent concentration. Detects a band of approximately 13 kDa.

    ターゲット情報

    • 機能

      Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

    • 組織特異性

      Brain and also in atria, but at much lower levels than ANP.

    • 配列類似性

      Belongs to the natriuretic peptide family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 別名

      • ANFB_HUMAN antibody
      • BNP antibody
      • BNP(1-32) antibody
      • BNP(5-29) antibody
      • BNP-32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Gamma-brain natriuretic peptide antibody
      • Natriuretic peptide precursor B antibody
      • nppb antibody
      • NT proBNP antibody
      see all

    画像

    • Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)
      Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)
      WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].

    参考文献 (1)

    ab13123 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13123 は 1 報の論文で使用されています。

    • Kim YS  et al. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol 103:4779-4788 (2019). PubMed: 31041470

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Inquiry: Dear Sir/Madam, I am looking for antibodies directed at or cross-reactive with rat NT-pro-BNP. Preferably monoclonal and source from any species. There are multiple monoclonal available on your site, but directed to human. Do you have antibodies that are crossreactive to rat. Best Regards,

    Read More
    Answer

    Thank you very much for your inquiry.

    I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

    I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

    We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

    AA 1-12 no homology
    AA 13-27 58%
    AA 28-45 62%
    AA 61-76 no homology.

    I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

    Read More

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