HRP Anti-Myc tag 抗体 (ab1326)
Key features and details
- HRP Rabbit polyclonal to Myc tag
- Suitable for: ELISA, WB, ICC
- Conjugation: HRP
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
HRP Anti-Myc tag antibody
Myc tag 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit polyclonal to Myc tag -
由来種
Rabbit -
標識
HRP -
特異性
In a direct ELISA against 1 ug of C-Myc peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate. -
アプリケーション
適用あり: ELISA, WB, ICCmore details -
免疫原
Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
(Peptide available asab13837) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.00
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Immunizing Peptide (Blocking)
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1326の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/5000 - 1/20000.
|
|
| WB | (3) |
1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
|
| ICC |
1/200 - 1/500.
|
| 特記事項 |
|---|
|
ELISA
1/5000 - 1/20000. |
|
WB
1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837). |
|
ICC
1/200 - 1/500. |
ターゲット情報
-
関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
別名
- c-myc tag antibody
- Myc Epitope Tag antibody
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (19)
ab1326 は 19 報の論文で使用されています。
- Xing J et al. Identification of poly(ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells. Nat Commun 12:2681 (2021). PubMed: 33976210
- Fan XJ et al. NONO phase separation enhances DNA damage repair by accelerating nuclear EGFR-induced DNA-PK activation. Am J Cancer Res 11:2838-2852 (2021). PubMed: 34249431
- Grigaitis R et al. Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis. Dev Cell 53:706-723.e5 (2020). PubMed: 32504558
- Zhang A et al. EphA2 phosphorylates NLRP3 and inhibits inflammasomes in airway epithelial cells. EMBO Rep 21:e49666 (2020). PubMed: 32352641
- Lee S et al. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-a-Induced Apoptosis via Different Routes. Cell Rep 26:2194-2211.e6 (2019). PubMed: 30784599