HRP Anti-mH2A1 抗体 [EPR9359(2)] (ab209320)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9359(2)] to mH2A1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-mH2A1 antibody [EPR9359(2)]
mH2A1 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EPR9359(2)] to mH2A1 -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, HeLa and HEK293 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9359(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-mH2A1 antibody [EPR9359(2)] (ab183041)
- Alexa Fluor® 488 Anti-mH2A1 antibody [EPR9359(2)] (ab208563)
- Alexa Fluor® 647 Anti-mH2A1 antibody [EPR9359(2)] (ab208879)
- Alexa Fluor® 555 Anti-mH2A1 antibody [EPR9359(2)] (ab211851)
- Alexa Fluor® 594 Anti-mH2A1 antibody [EPR9359(2)] (ab217090)
- Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (ab232602)
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab209320の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
ターゲット情報
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機能
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 histone H2A domain.
Contains 1 Macro domain. -
翻訳後修飾
Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin. -
細胞内局在
Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 9555 Human
- Entrez Gene: 26914 Mouse
- Entrez Gene: 29384 Rat
- Omim: 610054 Human
- SwissProt: O75367 Human
- SwissProt: Q9QZQ8 Mouse
- SwissProt: Q02874 Rat
- Unigene: 420272 Human
see all -
別名
- Core histone macro h2a.1 antibody
- Core histone macro-H2A.1 antibody
- H2A histone family member Y antibody
see all
画像
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All lanes : HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : H2AFY (macroH2A.1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 90 secondsab209320 was shown to recognize macroH2A.1 in wild-type HAP1 cells as signal was lost at the expected MW in H2AFY (macroH2A.1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and H2AFY (macroH2A.1) knockout samples were subjected to SDS-PAGE. Ab209320 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :HeLa whole cell lysate (ab150035)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209320 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab209320 は論文での使用が確認できていません。