HRP Anti-Human IgA 抗体 [1H9] (ab7383)

Submit a review Q&A (6)References (2)

Key features and details

  • HRP Mouse monoclonal [1H9] to Human IgA
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgM

製品の概要

  • 製品名

    HRP Anti-Human IgA antibody [1H9]
    Human IgA 一次抗体 製品一覧

  • 製品の詳細

    HRP Mouse monoclonal [1H9] to Human IgA

  • 由来種

    Mouse

  • 標識

    HRP

  • 特異性

    IgA Fc region.

  • 種交差性

    交差種: Human

  • 免疫原

    IgA from human colostrum.

  • 特記事項

    Concentration varies from lot to lot and can be provided on request.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.18% Dibasic monohydrogen sodium phosphate, 0.024% Monobasic dihydrogen potassium phosphate
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    1H9

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgM

  • 研究分野

ターゲット情報

  • 関連性

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • IgA1 antibody
      • Igh2 antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 (A2m marker) antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      see all

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab7383 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7383 は 2 報の論文で使用されています。

    • Memon B  et al. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Ther 9:15 (2018). PubMed: 29361979
    • Verdier J  et al. Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease. PLoS One 8:e62780 (2013). ELISA ; Human . PubMed: 23658774

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Question

    I want to detect human sIgA in ELISA form. Could I use Anti-Human IgA antibody [1H9] (HRP) (ab7383) for this purpose. Kindly advise.  

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    Answer

    Thank you for contacting us. The Anti-Human IgA antibody [1H9] (HRP), reference ab7383, was raised using human colostrum as immunogen and the colostrum contains mainly secretory IgA. I think this antibody is therefore suitable for your experiments. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Can you tell me what is the binding constant for these products? 1) ab7383 Human IgA antibody [1H9] (HRP) 2) ab7402 Human IgA antibody [A909] (HRP)

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    Answer

    Thank you for your enquiry. Unfortunately we have not determined the binding constants for these products, nor HRP-conjugation ratio. I am sorry for the inconvenience.

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    Question

    Can you tell me what is the concentration/ratio of HRP conjugation to antibody?

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    Answer

    Thank you for your enquiry. Unfortunately, the concentration/ratio of HRP conjugation to antibody was not determined. However, I can inform you that the antibodies were conjugated to Sigma Horseradish Peroxidase, Type VI (P 8375) by modification of the periodate method. (Wilsom, M.B., Nakane, P.K., “Immunofluorescense and Related Staining Techniques“, Elsevier/North Holland Biomedical Press, Amsterdam, 215 (1978)). I am sorry I can be of more help in this occasion but if there is anything else that I can assist you with, please do not hesitate to contact me.

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    Question

    Customer would like more details about the use of this antibody in ELISA.

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    Answer

    Thank you for your enquiry. We recommend using pair ab7401-ab7383 (capture-detection) for the detection of secretory IgA. You can use any general ELISA protocol and optimize it for the use with these antibodies. If you have any more questions, please contact us again.

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    Question

    Dear Ian, Can you please advice me when the following items will be shipped to us. - Item 1 (TIMP3 Antibody; cat# ab11814 ) from PO AX02134 - Item 16 (Human IgA Antibody [1H9]; cat# ab7383) from AX02125 Thanks for all your help. Kind Regards Ashrina Pratap Inventory & Shipping Manager

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    Answer

    Dear Ashrina We confirm that these items are being shipped today 1st Oct.

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    Question

    Customer would like to use these two antibodies in a sandwich ELISA. Ab7401 is coating, Ab7383 as conjugate; what should be used for detection?

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    Answer

    We recommend using pair ab7401-ab7383 (capture-detection) for the detection of secretory IgA. You can use any general ELISA protocol and optimize it for the use with these antibodies.

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