Application
ELISA
Sample
HIV Purified protein (purified from bacteria overexpression)
Specification
purified from bacteria overexpression
Type
Direct
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1 x PBST (0.5%Tween-20)
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
HIV-1 gag could be cleaved into MA, CA, NC, and P6. So here CANC is the combination of CA, and NC proteins, which can be easily over expressed in bacteria and purified for in vitro assays.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Shijian Zhang
Verified customer
投稿 Jun 03 2014