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HRP Anti-Hepatitis B Virus Surface Antigen 抗体 (ab19990)

Submit a review Q&A (2)

Key features and details

  • HRP Goat polyclonal to Hepatitis B Virus Surface Antigen
  • Suitable for: ELISA
  • Reacts with: Hepatitis B virus
  • Conjugation: HRP
  • Isotype: IgG

製品の概要

  • 製品名

    HRP Anti-Hepatitis B Virus Surface Antigen antibody
    Hepatitis B Virus Surface Antigen 一次抗体 製品一覧

  • 製品の詳細

    HRP Goat polyclonal to Hepatitis B Virus Surface Antigen

  • 由来種

    Goat

  • 標識

    HRP

  • アプリケーション

    適用あり: ELISAmore details

  • 種交差性

    交差種: Hepatitis B virus

  • 免疫原

    Full length native protein (purified) corresponding to Hepatitis B Virus Surface Antigen. Mixture of subtypes ad & ay.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab19990の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/1000.
特記事項
ELISA
1/1000.

ターゲット情報

  • 関連性

    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

  • 細胞内局在

    Virion membrane

  • 参照データベース

    • 別名

      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab19990 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab19990 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    I tried standard protocol (bicarb pH9.6 coating, blocking 1% BSA, then sandwich with your sAg standard, and detection with 2nd goat anti mouse HRP).

    I ordered this Ab pair you suggested. Can you pls give me the protocol details that have worked for Abcam? I'll be using the same sAg standard that I ordered before.

    Read More
    Answer

    Here are a few specific sELISA protocol details I was able to find:

    ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

    Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

    I hope this is helpful. Please contact me again if you have any further questions.

    Use our products? Submit an https://www.abcam.com/abreviews. Earn rewards!

    Read More

    Question

    Inquiry: I purchased two anti HBsAg antibodies (ab9193 and ab8636) and ab113610 protein as standard but don't see any signal in my ELISA attempt. Which antibody pairs have you tested for ELISA? Can you recommend a detection Ab that I can use with ab9193, e.g. HRP conjugated ab19990 or ab2042?

    Read More
    Answer



    If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

    We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

    We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

    Read More

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