HRP Anti-HA tag 抗体 (ab1190)

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ELISA - HRP Anti-HA tag antibody (ab1190)

    Key features and details

    • HRP Chicken polyclonal to HA tag
    • Suitable for: WB, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgY

    製品の概要

    • 製品名

      HRP Anti-HA tag antibody
      HA tag 一次抗体 製品一覧

    • 製品の詳細

      HRP Chicken polyclonal to HA tag

    • 由来種

      Chicken

    • 標識

      HRP

    • 特異性

      In a direct ELISA against 1 µg of HA peptide coupled to BSA as sample, conjugate diluted to 1/10,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.

    • アプリケーション

      適用あり: WB, ELISAmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. Corresponding to the influenza hemagglutinin-HA-epitope.
      Sequence:

      YPYDVPDYA

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.00
      Preservative: 0.002% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgY

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab1190 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.05 - 0.5 µg/ml.
    ELISA Use a concentration of 1 - 0.2 µg/ml.

    ターゲット情報

    • 関連性

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • 別名

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    画像

    • ELISA - HRP Anti-HA tag antibody (ab1190)
      ELISA - HRP Anti-HA tag antibody (ab1190)

      ELISA - Anti-HA tag antibody (HRP) (ab1190).

    参考文献 (5)

    ab1190 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1190 は 5 報の論文で使用されています。

    • Wang W  et al. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy 14:1574-1585 (2018). PubMed: 29940807
    • Scherer PC  et al. TRPV1 is a physiological regulator of µ-opioid receptors. Proc Natl Acad Sci U S A 114:13561-13566 (2017). PubMed: 29203659
    • van der Kant R  et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem 290:30280-90 (2015). PubMed: 26463206
    • Klein CJ  et al. Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons. Neurology 82:873-8 (2014). PubMed: 24500646
    • Tchekanda E  et al. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11:641-4 (2014). PubMed: 24747815

    レビューと Q&A

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    Question

    Hello, I am curious to konw if there is a clone name for this antibody? ab1190 - Chicken polyclonal to HA tag (HRP)

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    Answer

    Thank you for contacting us with your question. Since this antibody is a polyclonal, there is not a clone name associated with it. Only monoclonal antibodies will have a clone name. You can keep track of a polyclonal antibody based on their immunogen, host species, purity, or manufacturer and catalog number. I hope this information is helpful, but please let me know if you have any further questions.

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