Application
Western blot
Sample
Escherichia coli Recombinant protein (Expressed in E.Coli)
Loading amount
10 µg
Specification
Expressed in E.Coli
Gel Running Conditions
Reduced Denaturing (PVDF)
Blocking step
Block-it PBS-T as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Other product details
Dilution
1/2000
Incubation time
10 minute(s) · Temperature: 21°C · Diluent: Block-it PBS-T
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 28 kDa
Negative control
Well A12 contained no GST protein.
Additional data
Additional Notes
The proteins were expressed and purified using 96-well system. The eluted protein was then used for dot-blot experiment using PVDF membrane.
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投稿 Mar 27 2009