Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver and Testes whole and nuclear lysates)
Loading amount
50 µg
Specification
Liver and Testes whole and nuclear lysates
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Concentration
0.25 µg/ml
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS-T(0.1%)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 38 kDa Non-specific: 30-120 kDa
Positive control
Liver and testes samples from normal mouse tissue
Negative control
Molecular weight ladder standards
Additional data
Additional Notes
Nice clean band at ~38 kD in Liver cyto and nuclear, but lots of cross-reactive bands in testes cyto and nuclear lane, from ~30 kD to 120 kD.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Peter Thompson
Verified customer
投稿 Jan 24 2008