Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
50 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Blocking buffer
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~40 kDa kDa
Positive control
cytoplasmic fraction
Negative control
nuclear fraction
Additional data
Additional Notes
Cytoplasmic (C) and nuclear (N) protein extracts from fractionated cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ioannis Gavvovidis
Verified customer
投稿 Dec 15 2010