Application
Western blot
Sample
Human Cell lysate - whole cell (Human FOXP3 transfected COS cells)
Specification
Human FOXP3 transfected COS cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Negative control
vector control tranfected COS cells
Additional data
Additional Notes
Doesn't pick up any additional bands (including antibody bands when doing IPs).
Primary incubated in TBS with 0.3% BSA.
Primary incubated in TBS with 0.3% BSA.
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投稿 Jan 27 2006