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    hrp-fluorescein-antibody-ab6656.pdf

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HRP Anti-Fluorescein 抗体 (ab6656)

  • Datasheet
  • SDS
Reviews (2)Q&A (7)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (ab6656)
  • Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (ab6656)

Key features and details

  • HRP Goat polyclonal to Fluorescein
  • Suitable for: ELISA, IHC-P, IHC-Fr, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

こちらの製品もご検討ください

標識
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
一次抗体
Anti-Fluorescein antibody (ab19491)
クロモゲン
Product image
DAB Substrate Kit (ab64238)

関連製品

製品の概要

  • 製品名

    HRP Anti-Fluorescein antibody
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細

    HRP Goat polyclonal to Fluorescein
  • 由来種

    Goat
  • 標識

    HRP
  • 特異性

    This antibody reacts with FITC-conjugated proteins.
  • アプリケーション

    適用あり: ELISA, IHC-P, IHC-Fr, WBmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

関連製品

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6656の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/200000.
IHC-P (1)
1/500 - 1/2500.
IHC-Fr (1)
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
特記事項
ELISA
1/200000.
IHC-P
1/500 - 1/2500.
IHC-Fr
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.

ターゲット情報

  • 関連性

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (ab6656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (ab6656)This image is courtesy of an anonymous Abreview

    Immunohistochemical analysis of Mouse thymus in which the medullary epithellai cells are labelled with fluorescein-conjugated UEA1 lectin with ab6656. Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 40 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C.

    See Abreview

  • Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (ab6656)
    Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (ab6656)This image is courtesy of an anonymous Abreview

    ab6656 staining GFP in Mouse mammary carcinoma metastasis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Perfusion fixed tissue with 4% paraformaldehyde, blocked with 0.5% Perkin-Elmer TNB blocking reagent for 30 minutes at 25°C  and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500) for 30 minutes at 25°C

    Micrograph demonstrates use of ab6662 for detection of GFP-positive tumor cells (LEFT) and an adjacent section after amplification with ab6656 (RIGHT). Images were taken with identical settings on a Leica confocal microscope.

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (6)

ab6656 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6656 は 6 報の論文で使用されています。

  • Healing E  et al. A panel of colorimetric assays to measure enzymatic activity in the base excision DNA repair pathway. Nucleic Acids Res 47:e61 (2019). PubMed: 30869144
  • de Lucas M  et al. Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell-Type Proliferation and Differentiation. Plant Cell 28:2616-2631 (2016). In situ hybridization ; Arabidopsis thaliana . PubMed: 27650334
  • Lin WR  et al. The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis. Biomed Res Int 2016:4601532 (2016). PubMed: 27314021
  • Xiang D  et al. Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors. Theranostics 5:1083-97 (2015). IHC . PubMed: 26199647
  • Shigdar S  et al. The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One 8:e57613 (2013). IHC-P ; Human . PubMed: 23460885
  • Kett LR  et al. LRRK2 Parkinson disease mutations enhance its microtubule association. Hum Mol Genet 21:890-9 (2012). PubMed: 22080837

レビューと Q&A

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1-9 of 9 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fluorescein antibody (HRP)

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (thymus)
Antigen retrieval step
None
Permeabilization
Yes - Tween20
Specification
thymus
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 29 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Fluorescein antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse mammary carcinoma metastasis)
Specification
mouse mammary carcinoma metastasis
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 May 01 2009

Question

I am wondering whether this antibody (Anti-Fluorescein antibody HRP ab6656) can bind to other fluorescein derivative such as 6'-FAM. Any data upon which part of FITC is recognized by this antibody?

Read More

Abcam community

Verified customer

Asked on Dec 28 2012

Answer

Thank you for your inquiry and support of our products. The antibody is only tested and guaranteed against derivatives of FITC (Fluorescein isothiocyanate) but it may react with other forms as well. It has not been tested against 6’-FAM. The antibody is polyclonal and is expected to react with different portions of the molecule, but the specific binding sites are not characterized.

Read More

Abcam Scientific Support

Answered on Dec 28 2012

Question

We need an anti FITC –HRP conjugated antibody to to convert FITC fluorescence to a chromogenic substrate for light microscopy (e.g., DAB) in paraffin coupes of murine mammary glands .


Both ab 6656 and ab 19492 works on IHC-par among datasheet but which antibody would you advice us to use for our application?


Is there a difference in sensitivity because ab 19492 refers to a paper in 2011 who uses an additional tyramide signal amplification system and ab 6656 refers to a very short abreview without image saying it was difficult to find a good dilution but finally it worked.

Read More

Abcam community

Verified customer

Asked on Mar 01 2012

Answer

Thank you for contacting us.

Without a direct comparison, it is a little difficult to choose between the two. The ab6656 datasheet also has a review of IHC with TSA amplification on frozen tissue, after applying the antibody at a dilution of 1/500.

Neither antibody has received any complaints in the past year but I recommend ab6656 based on the dilutions recommended for the other applications, for instance 1/1000 - 1/5000 for western blotting versus 1/20 - 1/200 for ab19492. This suggests that ab6656 has higher affinity and/or HRP activity, but we cannot be sure without direct comparison using the same samples and supplementary reagents.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 01 2012

Question

Goodevening, my antibody ordered over to you (order #971173) just arrived (thank you for your efficiency by the way) and I was looking for the datasheet on your website since I was searching for the recommended concentration to use it in an IP and WB assay but I can't find this information: am I missing it or it's not there? Can you help me in any way? The antibody is ab6656 anti fluorescein. Thank you very much  

Read More

Abcam community

Verified customer

Asked on Oct 24 2011

Answer

Thank you for your enquiry. The concentration of this antibody is 1.000 mg/ml, however the working dilution/concentration needs to be determined since the signal depends on several factors (such as expression levels of the target, sample preparation etc.). I would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1/100-1/1000) and then adjust the dilution accordingly from the results obtained. I hope this helps and if I can assist further, please do not hesitate to contact me.      

Read More

Abcam Scientific Support

Answered on Oct 24 2011

Question

Will this antibody react with FITC too?

Read More

Abcam community

Verified customer

Asked on Aug 03 2011

Answer

Thank you for your phone call today and for your patience while I was in contact with the lab. The lab confirmed that ab6656 has been tested on FITC-conjugated proteins, and so the antibody is appropriate for your studies. I hope this information is helpful, but please let me know if you have any questions.  

Read More

Abcam Scientific Support

Answered on Aug 03 2011

Question

Hi. My lab works with a class of cell-permeable, sequence-specific, DNA-binding, synthetic small molecules. One of our active compounds has a fluorescein moiety on it. Would it be possible to perform ChIP or ChIP on chip with a fluorescein antibody? Thanks.

Read More

Abcam community

Verified customer

Asked on Dec 20 2005

Answer

Thank you for your enquiry. Yes, I would consider it highly likely that you will be able to use an anti-fluorescein antibody to immunoprecipitate cross linked chromatin associated with your small molecule. You may also wish to consider NChIP as this has been employed using proteins that directly bind DNA. Furthermore if the context of chromatin is not important you may wish to adopt a DNA IP approach, incubating your small molecule with purified DNA and then performing a modified IP approach. For further details of these approaches please see our protocols web page at: http://ops.abcam.com/index.html?pageconfig=popular_protocols Your assay may require some optimisation given a potential obscuring of the fluorescein epitope under cross linked conditions, but in theory there is no reason why this should not work. Unfortunately we do not have a fluorscein antibody that has been tested using ChIP. In this case I would recommend an antibody that has been characterized and demonstrated to work in as many applications as possible, in particular IP. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Dec 21 2005

Question

I just want to confirm that it is the correct procedure to use the HRP chromagen kit (AEC)after using the HRP conjugated fluorescein antibody?

Read More

Abcam community

Verified customer

Asked on Oct 01 2004

Answer

Thank you for your enquiry. Yes, the chromogen AEC kit is used after using the HRP conjugated fluorescein antibody in immunohistochemical techniques. If you are doing ELISA then a TMB kit is better.

Read More

Abcam Scientific Support

Answered on Oct 01 2004

Question

I've come across the product: anti-fluorescein conjugated to HRP (product code ab6656-100). Please would it be possible to know what is the specific activity (in units / mg product) of the conjugate? I've looked in the product datasheet but this important piece of information is missing.

Read More

Abcam community

Verified customer

Asked on Aug 22 2001

Answer

Sorry, we do not have any data on the specific activity of the HRP.

Read More

Abcam Scientific Support

Answered on Aug 22 2001

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