HRP Anti-Fluorescein 抗体 (ab19492)
Key features and details
- HRP Rabbit polyclonal to Fluorescein
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
製品の概要
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製品名
HRP Anti-Fluorescein antibody
Fluorescein 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit polyclonal to Fluorescein -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: IHC-P, WB, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Fluorescein coupled to KLH
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA -
Concentration information loading... -
特記事項（精製）
Solid phase hapten antibody capture. Affinity purified and absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated substitution ratio: HRP:IgG is >1.5 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19492の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
1/20 - 1/200.
|
|
| ELISA |
1/200 - 1/1000.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/20 - 1/200. |
|
ELISA
1/200 - 1/1000. |
ターゲット情報
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関連性
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
別名
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (ab19492)Image from Jourdain E et al., PLoS One. 2011;6(9):e24155. Epub 2011 Sep 1. Fig 1.; doi:10.1371/journal.pone.0024155; September 1, 2011, PLoS ONE 6(9): e24155.
Immunohistochemical analysis of chicken trachea tissue, infected with an avian influenza virus, staining Fluorescein with ab19492.
Fluoroscein-labeled influenza viruses were incubated with tissue overnight at 4°C. Sections were incubated with primary antibody and the signal was amplified with a tyramide signal amplification system.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab19492 は 7 報の論文で使用されています。
- Eriksson P et al. Attachment Patterns of Human and Avian Influenza Viruses to Trachea and Colon of 26 Bird Species - Support for the Community Concept. Front Microbiol 10:815 (2019). PubMed: 31057520
- Eriksson P et al. Characterization of avian influenza virus attachment patterns to human and pig tissues. Sci Rep 8:12215 (2018). PubMed: 30111851
- Hasegawa K et al. Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. PLoS One 12:e0172030 (2017). PubMed: 28182792
- Kraaij MD et al. The NOX2-mediated ROS producing capacity of recipient cells is associated with reduced T cell infiltrate in an experimental model of chronic renal allograft inflammation. Transpl Immunol 30:65-70 (2014). PubMed: 24365514
- Jourdain E et al. The Pattern of Influenza Virus Attachment Varies among Wild Bird Species. PLoS One 6:e24155 (2011). IHC-P . PubMed: 21909418
- Rong YP et al. The BH4 domain of Bcl-2 inhibits ER calcium release and apoptosis by binding the regulatory and coupling domain of the IP3 receptor. Proc Natl Acad Sci U S A 106:14397-402 (2009). IP . PubMed: 19706527
- Mezo M et al. Evaluation of the flukicide treatment policy for dairy cattle in Galicia (NW Spain). Vet Parasitol 157:235-43 (2008). ELISA . PubMed: 18774648