HRP Anti-CD90 / Thy1 抗体 [MRC OX-7] (ab199219)
Key features and details
- HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-CD90 / Thy1 antibody [MRC OX-7]
CD90 / Thy1 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1 -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Rabbit, Guinea pig -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Rat brain tissue lysate and PC12 whole cell lysate.
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特記事項
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MRC OX-7 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
- PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199219の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
Observed molecular weight may vary depending on the glycosylation level of the target. |
特記事項 |
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WB
1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa). Observed molecular weight may vary depending on the glycosylation level of the target. |
ターゲット情報
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機能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
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別名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
画像
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All lanes : HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219) at 1/5000 dilution
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab199219 は論文での使用が確認できていません。