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HRP Anti-Cardiac Troponin I 抗体 [MF4] (ab10239)

Submit a review Q&A (2)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)

    Key features and details

    • HRP Mouse monoclonal [MF4] to Cardiac Troponin I
    • Suitable for: IHC-P
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Unconjugated

    製品の概要

    • 製品名

      HRP Anti-Cardiac Troponin I antibody [MF4]
      Cardiac Troponin I 一次抗体 製品一覧

    • 製品の詳細

      HRP Mouse monoclonal [MF4] to Cardiac Troponin I

    • 由来種

      Mouse

    • 標識

      HRP

    • 特異性

      This antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation. This antibody does not cross-react with skeletal muscle troponin I. Using multiple combinations of MAbs antibodies with different epitope specificity as capture and multiple MAbs as detection, the sensitivity of the sandwich ELISA can be increased 2-4 fold.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      非交差種: Rabbit, Fish

    • 免疫原

      free human cardiac troponin and/or native troponin complex.

    • エピトープ

      190-196 aa

    • ポジティブ･コントロール

      • IHC-P: Human heart FFPE tissue sections.

    • 特記事項

      Concentration varies from lot to lot and can be provided on request.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab10239の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

    • 関連疾患

      Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
      Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

    • 配列類似性

      Belongs to the troponin I family.
    • Information by UniProt

    • 参照データベース

      • 別名

        • cardiac muscle antibody
        • Cardiac troponin I antibody
        • cardiomyopathy, dilated 2A (autosomal recessive) antibody
        • Cardiomyopathy, familial hypertrophic, 7, included antibody
        • CMD1FF antibody
        • CMD2A antibody
        • CMH7 antibody
        • cTnI antibody
        • Familial hypertrophic cardiomyopathy 7 antibody
        • MGC116817 antibody
        • RCM1 antibody
        • Tn1 antibody
        • Tni antibody
        • TNN I3 antibody
        • TNNC 1 antibody
        • TNNC1 antibody
        • TNNI3 antibody
        • TNNI3_HUMAN antibody
        • Troponin I antibody
        • Troponin I cardiac antibody
        • Troponin I cardiac muscle antibody
        • Troponin I cardiac muscle isoform antibody
        • Troponin I type 3 cardiac antibody
        • troponin I, cardiac 3 antibody
        • TroponinI antibody
        • Ttroponin I type 3 (cardiac) antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)

        IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10239, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      参考文献 (2)

      ab10239 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab10239 は 2 報の論文で使用されています。

      • Nakano SJ  et al. Increased myocyte calcium sensitivity in end-stage pediatric dilated cardiomyopathy. Am J Physiol Heart Circ Physiol 317:H1221-H1230 (2019). WB . PubMed: 31625780
      • Perrone CE  et al. Methionine restriction effects on mitochondrial biogenesis and aerobic capacity in white adipose tissue, liver, and skeletal muscle of F344 rats. Metabolism 59:1000-11 (2010). WB ; Rat . PubMed: 20045141

      レビューと Q&A

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      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      Thank you for your helpful reply. Regarding Cardiac Troponin I protein ref. ab9936, I see that it has been only tested for WB. Do you have information using it for ELISA? In other case, do you have a specific one for ELISA?  Regards, 

      Read More
      Answer

      Thank you for your email. The ab9936 product is a peptide which can be used to block antibodies ab7829, ab8290 and ab8291. This peptide can be used to block these antibodies irrespective of applications e.g. It is suitable to use with antibodies in Immunoprecipitation, western blot, Immunohistochemistry, ELSIA & also other blocking assays. I hope this information will be helpful. Should you have any other question please do not hesitate to ask.

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      Question

      Dear Sir or Madam, My name is XXXX, and I am a biologist of a biotech company called STAT-Diagnostica, which is located in the Parc Científic de Barcelona (Spain). I am interested in performing a Direct Sandwich ELISA assay and I would like to consult some questions regarding the selection of antibodies and human troponin protein. I have seen that you recommend anti-cardiac troponin I antibody [284 (19C7)]-ref. ab10236 due to their high sensitivity, good kinetics, low background and high reproducibility. I would use it as capture antibody: - could you please tell me what is the recommended concentration for coating the well plates?, and also - what would be the optimum pH of the coating buffer for this antibody, 9,6 or 7.4? On the other hand, detection antibodies could be anti-cardiac troponin I antibody [MF4]-HRP-ref.ab10239 and anti-cardiac troponin antibody [284(19C7)]-ref.ab19615. Is it correct? Could you tell me what would approximately be the optimal assay concentration? Furthermore, I would like to use Cardiac Troponin I protein ref. ab9936 as a standard, but I see that It has been only tested for WB. Do you have information about using it for ELISA? In other case, do you have a specific one for ELISA? Lastly, I would like to know what would be the delivery time. I would appreciate your comments. Many thanks in advance. Best Regards,

      Read More
      Answer

      Thank you for your email. We have updated the datasheet of ab10236; few of the match pairs are no longer available in our catalogue so we have removed these from the datasheet. We recommend using ab10236 as a capture ab along with ab10239 as a detection antibody. In general many general protocols could be used with our cTnI antibodies and the optimal working concentrations should be determined separately for each application by the user. In our own lab we are using a special two-step immunofluorometric assay and streptavidin-coated plates with biotinylated capture MAbs and detection MAbs which are conjugated with stable europium chelate. The MAb concentrations in this kind of assay are different than what are needed in some other kind of assay. With very general protocol using PBS buffers and HRP detection method I can only recommend to start testing with something like 5 ug/ml MAb concentration for capture MAb and 0.5-1 ug/ml concentration for HRP-conjugated detection MAb and then to start optimization from there. Regarding pH I would recommend 7.4. I hope that this information is helpful to you. Should you have any question please do not hesitate to contact me.

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