Anti-HRP 抗体 (ab2116)

Submit a review Q&A (2)References (1)

Key features and details

  • Sheep polyclonal to HRP
  • Suitable for: WB, IP, ELISA
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-HRP antibody
    HRP 一次抗体 製品一覧

  • 製品の詳細

    Sheep polyclonal to HRP

  • 由来種

    Sheep

  • アプリケーション

    適用あり: WB, IP, ELISAmore details

  • 免疫原

    Horseradish peroxidase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    IgG fraction

  • 特記事項（精製）

    IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ELISA
  • 追加情報
    Optimal titers for applications should be determined by the researcher.

    • ターゲット情報

    • 関連性

      Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

    • 細胞内局在

      Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

    • 別名

      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab2116 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2116 は 1 報の論文で使用されています。

    • Li YF  et al. Targeting MRP4 expression by anti-androgen treatment reverses MRP4-mediated docetaxel resistance in castration-resistant prostate cancer. Oncol Lett 14:1748-1756 (2017). PubMed: 28789405

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    is this antiserum suitable for inhibiting peroxidase activity?

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    Answer

    The antibody has not been tested for inhibition of peroxidase. We have introduced a new scheme to allow researchers to try an antibody in an unknown application 'risk free'. If you want to place an order with our reference LD/NS.02.08.01 we can include the antibody in this scheme (No#2 below applies in this case). 1.) Abcam will refund the product if it does not work in the application and species as stated on the datasheet. 2.) If it is used in an application or on a species that is not listed on the datasheet then Abcam will refund or replace the product for another of equal value as long as Abcam receives the negative data for publishing on the datasheets to help other researchers.

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    Question

    I have tumours grown using transfectants stably expressing the HRP enzyme and would like to stain using immunohistochemistry. Are any of your horseradish peroxidase antibodies suitable for IHC in either frozen or paraffin embeded sections, and would they also pick up other peroxidases present in the tissue such as myeloperoxidase?

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    Answer

    We do not have any reports of any of our HRP antibodies being used as a primary antibody with frozen or paraffin embedded sections. In addition, cross-reactivity with endogenous tissue peroxidases has not been tested.

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