HRP Anti-6X His tag® 抗体 (ab3553)
Key features and details
- HRP Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgY
製品の概要
-
製品名
HRP Anti-6X His tag® antibody
6X His tag® 一次抗体 製品一覧 -
製品の詳細
HRP Chicken polyclonal to 6X His tag® -
由来種
Chicken -
標識
HRP -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
-
特記事項
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
ミエローマ
unknown -
アイソタイプ
IgY -
軽鎖の種類
unknown -
研究分野
関連製品
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3553の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. 1/1000.
|
| ELISA |
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. 1/1000. |
|
ELISA
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification. |
ターゲット情報
-
関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab3553 は 8 報の論文で使用されています。
- Chan CH et al. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. Int J Mol Sci 22:N/A (2021). PubMed: 33498219
- Manning JA et al. Dietary sodium modulates nephropathy in Nedd4-2-deficient mice. Cell Death Differ 27:1832-1843 (2020). PubMed: 31802037
- Burnside D et al. In Silico Engineering of Synthetic Binding Proteins from Random Amino Acid Sequences. iScience 11:375-387 (2019). PubMed: 30660105
- Miao J et al. Wnt/ß-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell 18:e13004 (2019). PubMed: 31318148
- Dragovic SM et al. Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. Cell Host Microbe 23:523-535.e5 (2018). PubMed: 29649443
- Zhang K et al. Distinct expression profile and histological distribution of NLRP3 inflammasome components in the tissues of Hainan black goat suggest a site-specific role in the inflammatory response. Acta Vet Hung 65:402-416 (2017). PubMed: 28956480
- Henshall TL et al. Deletion of Nedd4-2 results in progressive kidney disease in mice. Cell Death Differ 24:2150-2160 (2017). IHC-P ; Mouse . PubMed: 28862701
- Allan CM et al. A conserved START domain coenzyme Q-binding polypeptide is required for efficient Q biosynthesis, respiratory electron transport, and antioxidant function in Saccharomyces cerevisiae. Biochim Biophys Acta 1831:776-791 (2013). PubMed: 23270816