HRP Anti-6X His tag® 抗体 (ab3553)

Reviews (1)Q&A (1)References (7)

Key features and details

  • HRP Chicken polyclonal to 6X His tag®
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgY

製品の概要

  • 製品名

    HRP Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    HRP Chicken polyclonal to 6X His tag®

  • 由来種

    Chicken

  • 標識

    HRP

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • ミエローマ

    unknown

  • アイソタイプ

    IgY

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3553の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. 1/1000.
ELISA
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.
特記事項
WB
Use at an assay dependent concentration. 1/1000.
ELISA
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献 (7)

ab3553 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3553 は 7 報の論文で使用されています。

  • Chan CH  et al. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. Int J Mol Sci 22:N/A (2021). PubMed: 33498219
  • Manning JA  et al. Dietary sodium modulates nephropathy in Nedd4-2-deficient mice. Cell Death Differ 27:1832-1843 (2020). PubMed: 31802037
  • Burnside D  et al. In Silico Engineering of Synthetic Binding Proteins from Random Amino Acid Sequences. iScience 11:375-387 (2019). PubMed: 30660105
  • Miao J  et al. Wnt/ß-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell 18:e13004 (2019). PubMed: 31318148
  • Dragovic SM  et al. Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. Cell Host Microbe 23:523-535.e5 (2018). PubMed: 29649443
  • Henshall TL  et al. Deletion of Nedd4-2 results in progressive kidney disease in mice. Cell Death Differ 24:2150-2160 (2017). IHC-P ; Mouse . PubMed: 28862701
  • Allan CM  et al. A conserved START domain coenzyme Q-binding polypeptide is required for efficient Q biosynthesis, respiratory electron transport, and antioxidant function in Saccharomyces cerevisiae. Biochim Biophys Acta 1831:776-791 (2013). PubMed: 23270816

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Western blot abreview for Anti-6X His tag® antibody (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (protein produced in mammalian expression system)
Loading amount
1 µg
Specification
protein produced in mammalian expression system
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Nov 29 2007

Question

Does the chicken anti-His tag polyclonal antibody bind to N-terminal His tags?

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Answer

Thank you for your enquiry. Yes, this product will detect N-terminal HIS tags.

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