HRP Anti-6X His tag® 抗体 (ab1269)
Key features and details
- HRP Goat polyclonal to 6X His tag®
- Suitable for: WB, ELISA, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
製品の概要
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製品名
HRP Anti-6X His tag® antibody
6X His tag® 一次抗体 製品一覧 -
製品の詳細
HRP Goat polyclonal to 6X His tag® -
由来種
Goat -
標識
HRP -
アプリケーション
適用あり: WB, ELISA, ICCmore details -
種交差性
交差種: Species independent -
免疫原
-
特記事項
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1269の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000 - 1/30000.
|
| ELISA | (1) |
1/10000 - 1/100000.
|
| ICC |
1/200 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/30000. |
|
ELISA
1/10000 - 1/100000. |
|
ICC
1/200 - 1/500. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab1269 は 7 報の論文で使用されています。
- Xu X et al. Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump. Elife 9:N/A (2020). PubMed: 32459174
- Lee CH et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
- Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283
- Seijsing J et al. Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP. PLoS One 8:e81350 (2013). ELISA . PubMed: 24260574
- de Vrij J et al. A cathepsin-cleavage site between the adenovirus capsid protein IX and a tumor-targeting ligand improves targeted transduction. Gene Ther 19:899-906 (2012). PubMed: 22011643
- Jevnikar Z et al. Cathepsin X cleavage of the {beta}2 integrin regulates talin-binding and LFA-1 affinity in T cells. J Leukoc Biol 90:99-109 (2011). PubMed: 21454358
- Uil TG et al. A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibers. J Gene Med 11:990-1004 (2009). ELISA . PubMed: 19757488