HRP Anti-6X His tag® 抗体 (ab1269)

Reviews (3)Q&A (1)References (7)

Key features and details

  • HRP Goat polyclonal to 6X His tag®
  • Suitable for: WB, ELISA, ICC
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

製品の概要

  • 製品名

    HRP Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    HRP Goat polyclonal to 6X His tag®

  • 由来種

    Goat

  • 標識

    HRP

  • アプリケーション

    適用あり: WB, ELISA, ICCmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1269の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000 - 1/30000.
ELISA (1)
1/10000 - 1/100000.
ICC
1/200 - 1/500.
特記事項
WB
1/1000 - 1/30000.
ELISA
1/10000 - 1/100000.
ICC
1/200 - 1/500.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献 (7)

ab1269 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1269 は 7 報の論文で使用されています。

  • Xu X  et al. Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump. Elife 9:N/A (2020). PubMed: 32459174
  • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283
  • Seijsing J  et al. Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP. PLoS One 8:e81350 (2013). ELISA . PubMed: 24260574
  • de Vrij J  et al. A cathepsin-cleavage site between the adenovirus capsid protein IX and a tumor-targeting ligand improves targeted transduction. Gene Ther 19:899-906 (2012). PubMed: 22011643
  • Jevnikar Z  et al. Cathepsin X cleavage of the {beta}2 integrin regulates talin-binding and LFA-1 affinity in T cells. J Leukoc Biol 90:99-109 (2011). PubMed: 21454358
  • Uil TG  et al. A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibers. J Gene Med 11:990-1004 (2009). ELISA . PubMed: 19757488

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-6X His tag® antibody (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Mar 17 2010

Question

Customer using Goat polyclonal to 6X His tag for ELISA. Wants to coat nickle plate with this antibody. Then detect a mutant protein with his tag on (no antibodies against this protein). Will antibody still recognise bound protien in ELISA?

Read More
Answer

I feel the best option would be for you to bind your mutant protein to the nickel plate. The ab1269 antibody can then be used to detect the his-tag on your mutant protein. It then leaves the HRP on the antibody free for detection. If you coat the plate with the ab1269 antibody, it will bind your mutant protein, but without a secondary antibody, you may have no means of detecting this.

Read More

ELISA abreview for Anti-6X His tag® antibody (HRP)

 Good
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Recombinant protein
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: PBS 10% FC
Type
Direct
Read More

Dr. Michael Aigner

Verified customer

投稿 Aug 15 2005

Western blot abreview for Anti-6X His tag® antibody (HRP)

 Good
Abreviews
Application
Western blot
Sample
Human Recombinant protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Mr. Sunil Verma

Verified customer

投稿 Aug 05 2005

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