Application
ELISA
Sample
Escherichia coli Purified protein (BL21(DE3))
Specification
BL21(DE3)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Sandwich (Capture)
Other product details
Dilution
1/5000
Incubation time
1 hour(s) and 30 minute(s) · Diluent: TBST+5%Milk
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Coated plate with antigen, then applied our purified protein. Finally detected by anti-his antibody from Abcam.
Row A: Sample
Row B: Negative control
Row A: Sample
Row B: Negative control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 27 2009