Name: HRP Anti-6X His tag® antibody
SKU: ab1187
Rating: 4 (12 reviews)

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Western blot - HRP Anti-6X His tag® antibody (ab1187)

Key features and details

HRP Rabbit polyclonal to 6X His tag®
Suitable for: ELISA, WB
Reacts with: Species independent
Conjugation: HRP
Isotype: IgG

医薬用外毒物

製品名

HRP Anti-6X His tag® antibody
6X His tag® 一次抗体製品一覧
製品の詳細

HRP Rabbit polyclonal to 6X His tag®
由来種

Rabbit
標識

HRP
アプリケーション

適用あり: ELISA, WBmore details
種交差性

交差種: Species independent
免疫原

Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.

Run BLAST with Run BLAST with
ポジティブ･コントロール
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) can be used as a positive control in WB.
特記事項

Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor^® 488 (ab150077).

See other anti-rabbit secondary antibodies that can be used with this antibody.
HIS-TAG^® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.00
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
Concentration information loading...
精製度

Immunogen affinity purified
特記事項（精製）

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
ポリ/モノ

ポリクローナル
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab1187の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ELISA	(3)	1/5000 - 1/20000.
WB	(9)	1/1000 - 1/5000.

特記事項
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000.

関連性

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
細胞内局在

Depends upon the localization of the parent protein tagged with hexahistidine.
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
- HHHHHH tag antibody
- His tag antibody
- Polyhistidine Tag antibody
see all

Western blot - HRP Anti-6X His tag® antibody (ab1187)This image is courtesy of an Abreview submitted by Mr Ohrt Thomas

All lanes : HRP Anti-6X His tag® antibody (ab1187) at 1/4000 dilution

Lane 1 : 4ug Human His-tagged protein purified from E.coli
Lane 2 : 2ug Human His-tagged protein purified from E.coli
Lane 3 : 1ug Human His-tagged protein purified from E.coli
Lane 4 : 0.5ug Human His-tagged protein purified from E.coli

Observed band size: 136 kDa why is the actual band size different from the predicted?

See Abreview

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab1187 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1187 は 83 報の論文で使用されています。

Nürenberg-Goloub E et al. Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex. EMBO J 39:e103788 (2020). PubMed: 32064661
Jalal AS et al. ParB spreading on DNA requires cytidine triphosphate in vitro. Elife 9:N/A (2020). PubMed: 32077854
Constantinescu-Bercu A et al. Activated aIIbß3 on platelets mediates flow-dependent NETosis via SLC44A2. Elife 9:N/A (2020). PubMed: 32314961
Rai K et al. Enhanced anticoagulant activity of hirudin-i analogue co-expressed with arylsulfotransferase in periplasm of E. coli BL21(DE3). J Biotechnol 323:107-112 (2020). PubMed: 32795502
Kralovicova J et al. Cancer-Associated Substitutions in RNA Recognition Motifs of PUF60 and U2AF65 Reveal Residues Required for Correct Folding and 3' Splice-Site Selection. Cancers (Basel) 12:N/A (2020). PubMed: 32664474

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 17 Abreviews or Q&A

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)

Loading amount

25 µg

Specification

mouse pancreatic cancer primary cell lines

Gel Running Conditions

Reduced Denaturing

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Feb 09 2011

Application

Western blot

Sample

Human Recombinant protein (Overexpressed his-tagged protein in a eColi cell c)

Gel Running Conditions

Reduced Denaturing

Loading amount

1 µg

Specification

Overexpressed his-tagged protein in a eColi cell c

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Aug 13 2020

Application

Western blot

Loading amount

0.1 µg

Gel Running Conditions

Reduced Denaturing

Sample

Human Recombinant protein (purification sur colonne HIS Trap)

Specification

purification sur colonne HIS Trap

Blocking step

caséine as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C

Abcam user community

Verified customer

投稿 May 30 2013

Application

Western blot

Sample

Human Purified protein (purified His-tagged lipase, SPINK1)

Loading amount

1 µg

Specification

purified His-tagged lipase, SPINK1

Gel Running Conditions

Reduced Denaturing (15%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Miklos Sahin-Toth

Verified customer

投稿 Aug 12 2010

Application

Western blot

Sample

Mouse Cell lysate - whole cell (neuronal cell)

Loading amount

20 µg

Specification

neuronal cell

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

投稿 Feb 09 2010

Application

Western blot

Sample

Human Purified protein (Purified His-tagged extracellular human LDLR)

Loading amount

0.1 µg

Specification

Purified His-tagged extracellular human LDLR

Gel Running Conditions

Reduced Denaturing (4-20% gel)

Blocking step

Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Aug 25 2009

Application

ELISA

Sample

Escherichia coli Purified protein (BL21(DE3))

Specification

BL21(DE3)

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Type

Sandwich (Capture)

Abcam user community

Verified customer

投稿 Jan 27 2009

Application

Western blot

Sample

Escherichia coli Purified protein (BL21(DE3))

Loading amount

0.01 µg

Specification

BL21(DE3)

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Dec 30 2008

Application

Western blot

Sample

Human Purified protein (recombinant human fibronectin domain expressed in)

Loading amount

1 µg

Specification

recombinant human fibronectin domain expressed in

Gel Running Conditions

Reduced Denaturing (12% BisTris NuPAGE gel)

Blocking step

Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Sep 09 2008

Question

Can this antibody be stored at -20C?

Abcam community

Verified customer

Asked on Dec 01 2011

Answer

Thank you for calling Abcam earlier. After having spoken to the lab, I would advise you not to store ab1187 at at -20C as it will cause the product to lose some functionality due to the HRP conjugation.

Abcam Scientific Support

Answered on Dec 01 2011

1-10 of 17 Abreviews or Q&A

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For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

標識

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

アイソタイプ

研究分野

関連製品

Positive Controls

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

関連性

細胞内局在

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (83)

レビューと Q&A

Western blot abreview for Anti-6X His tag® antibody (HRP)

Western blot abreview for Anti-6X His tag® antibody (HRP)

Western blot

Western blot abreview for Anti-6X His tag® antibody (HRP)

Western blot abreview for Anti-6X His tag® antibody (HRP)

Western blot abreview for Anti-6X His tag® antibody (HRP)

ELISA abreview for Anti-6X His tag® antibody (HRP)

Western blot abreview for Anti-6X His tag® antibody (HRP)

Western blot abreview for Anti-6X His tag® antibody (HRP)