Application
Western blot
Sample
Human Cell lysate - other (human foreskin keratinocytes)
Loading amount
10 µg
Specification
human foreskin keratinocytes
Gel Running Conditions
Reduced Denaturing (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in 5% non fat milk
Secondary antibody
Name
Non-Abcam antibody was used: Sigma mouse secondary antibody
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 12 kDa
Positive control
4 donor primary human foreskin keratinocytes cell lines stable expressing the HPV 18 genome.
Negative control
Primary human forskin keratinocytes without the HPV 18 genome.
Additional data
Additional Notes
Legend for western blot
1- Primary foreskin keratinocytes
2- Donor 1 primary forskin keratinocytes containing HPV 18 genome
3- Donor 2 primary forskin keratinocytes containing HPV 18 genome
4- Donor 3 primary forskin keratinocytes containing HPV 18 genome
5- Donor 4 primary forskin keratinocytes containing HPV 18 genome
1- Primary foreskin keratinocytes
2- Donor 1 primary forskin keratinocytes containing HPV 18 genome
3- Donor 2 primary forskin keratinocytes containing HPV 18 genome
4- Donor 3 primary forskin keratinocytes containing HPV 18 genome
5- Donor 4 primary forskin keratinocytes containing HPV 18 genome
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 12 2012