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Anti-HPV18 E2 抗体 [2E7] (ab100969)

Submit a review Submit a question References (2)
Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (ab100969)

Key features and details

  • Mouse monoclonal [2E7] to HPV18 E2
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Human papillomavirus
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-HPV18 E2 antibody [2E7]

  • 製品の詳細

    Mouse monoclonal [2E7] to HPV18 E2

  • 由来種

    Mouse

  • 特異性

    Reacts in WB, and ELISA with HPV18 native E2 protein as well recombinant E2 protein expressed in bacteria and Cos 7 cells.

  • アプリケーション

    適用あり: WB, ELISA, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human papillomavirus

  • 免疫原

    Recombinant full length protein corresponding to HPV18 E2.

  • エピトープ

    Mapped to amino acids 1-83.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified from bacteria by affinity chromatography.

  • ポリ/モノ

    モノクローナル

  • クローン名

    2E7

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab100969の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA
Use a concentration of 0.1 µg/ml.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA
Use a concentration of 0.1 µg/ml.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 関連性

    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.

  • 細胞内局在

    Host nucleus

  • 参照データベース

    • 別名

      • E2 antibody
      • Human papillomavirus type 18 E2 protein antibody
      • Regulatory protein E2 antibody

    画像

    • Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
      Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
      All lanes : Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution

      Lane 1 : lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein
      Lane 2 : lysates of plain U20S cells
      Lane 3 : lysates of plain HeLa cells
      Lane 4 : lysates of U20S transfected with plasmid expressing HPV11 E2 protein
      Lane 5 : marker

      Predicted band size: 41 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (ab100969)
      Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (ab100969)
      Immunofluorescence (IF) analysis of HPV18 E2 protein in U2OS cells. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E2 detected by ab100969.

    参考文献 (2)

    ab100969 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab100969 は 2 報の論文で使用されています。

    • Fuentes-González AM  et al. Intratype variants of the E2 protein from human papillomavirus type 18 induce different gene expression profiles associated with apoptosis and cell proliferation. Arch Virol 164:1815-1827 (2019). PubMed: 30631960
    • Cruz-Gregorio A  et al. Human Papillomavirus Types 16 and 18 Early-expressed Proteins Differentially Modulate the Cellular Redox State and DNA Damage. Int J Biol Sci 14:21-35 (2018). WB . PubMed: 29483822

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