Anti-HPV16 L1 抗体 [CamVir 1] (ab69)

Submit a review Q&A (13)References (17)

Key features and details

  • Mouse monoclonal [CamVir 1] to HPV16 L1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human papillomavirus
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-HPV16 L1 antibody [CamVir 1]
    HPV16 L1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CamVir 1] to HPV16 L1

  • 由来種

    Mouse

  • 特異性

    The antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. The antibody reacts very strongly with biopsy specimins containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. Cross-reacts with HPV37.

  • アプリケーション

    適用あり: WB, IP, IHC-Pmore details

  • 種交差性

    交差種: Human papillomavirus

  • 免疫原

    Human papilloma virus type 16, major capsid protein L1.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

  • 細胞内局在

    Virion. Host Nucleus.

  • 参照データベース

    • 別名

      • HPV-16 antibody
      • HPV-16 capsid antibody
      • HPV16 capsid protein antibody
      • HPV16 L1 antibody
      • HPV16 major capsid protein L1 antibody
      • Human papillomavirus type 16 L1 antibody
      • Human papillomavirus type 16 major capsid protein L1 antibody
      • L1 antibody
      • Major capsid L1 protein antibody
      • Major capsid protein antibody
      • Major capsid protein L1 antibody
      see all

    参考文献 (17)

    ab69 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab69 は 17 報の論文で使用されています。

    • Karimi H  et al. Combination therapy using human papillomavirus L1/E6/E7 genes and archaeosome: a nanovaccine confer immuneadjuvanting effects to fight cervical cancer. Sci Rep 10:5787 (2020). PubMed: 32238821
    • Mboumba Bouassa RS  et al. Serum and cervicovaginal IgG immune responses against a7 and a9 HPV in non-vaccinated women at risk for cervical cancer: Implication for catch-up prophylactic HPV vaccination. PLoS One 15:e0233084 (2020). PubMed: 32421735
    • Xv Z  et al. Effective Neutralizing Antibody Produced in Mice Directly Immunized with Integrated Pichia pastoris Expressing HPV16L1 Protein. Viral Immunol 32:308-317 (2019). PubMed: 31381497
    • Day PM  et al. Interferon Gamma Prevents Infectious Entry of Human Papillomavirus 16 via an L2-Dependent Mechanism. J Virol 91:N/A (2017). WB . PubMed: 28250129
    • Grant BD  et al. A paper-based immunoassay to determine HPV vaccination status at the point-of-care. Vaccine 34:5656-5663 (2016). PubMed: 27667331
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Question

    Thank you very much for the information.
    I am however looking for the binding site of your camVir1 antibody. That is against HPV16 L1. Do you have a paper on that?
    Thanking you for your help

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately, we do not have any other information about the binding site, I am afraid!. The CamVir1 clone is a published clone, please check the literature if anybody else have published any more details about the epitope.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Product code: 69
    Inquiry: Hi, Which epitope on L1 does camvir1 bind to? Thanks Inga

    Read More
    Answer

    Thank you for contacting us.

    The exact epitope sequence has been mapped. The immunogen is a gel-purified HPV-18 E6-b galactosidase fusion protein.

    Please check the link below of original publication;

    http://vir.sgmjournals.org/content/68/5/1351.full.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    What is the epitope of antibody ab69?

    Read More
    Answer

    Thank you for contacting us.

    Please find attached the requested paper.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear technical team,

    Our customer is going to purchase ab69 and she would like to know if it has cross-reactivity to other HPV types except type 16.
    Please let me know.

    Best regards

    Read More
    Answer

    Thank you for contacting us.

    The anti-HPV16 L1 antibody [CamVir 1] (ab69) has shown some cross reactivity with other HPV types other than 16.The antibody reacts very strongly with biopsy specimens containing HPV-16 or -33, whilstvery weak reactionshave occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. There has also been cross reactivity observed with HPV37.

    I hope this information has been of help. If you have any other questions, please do not hesitate to ask.

    Read More

    Question

    Hola de nuevo Abcam:
    Uno de nuestros clientes adquirió estos 3 anticuerpos con nosotros, ellos los alicuotaron y almacenaron a -20°C, ahora que tratan de usarlos ninguno de los tres funciona, nos podrán ayudar, aún están dentro de los 6 meses de abpromise?
    AB10419 LOTE XXXXXXXX
    AB69 LOTE XXXXXXXXXX
    AB10505 LOTE XXXXXXXXX
    Muchas Gracias.¡

    Read More
    Answer

    Gracias por contactarnos de nuevo.

    ¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

    ¿En qué aplicación y especies se están usando?

    Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

    Gracias por la colaboración. Quedo a la espera de tu respuesta.

    Read More

    Question

    Thank you what is the status of our order?

    Read More
    Answer

    Unfortunately shipment of this order is being held up by our accounts team.

    Our accounting department can be contacted by email at mailto:us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to theorder reference numberin any correspondence with our accounting department.

    I hope this is helpful. Please contact me again with any technical questions you may have.

    Read More

    Question

    I purchased your mouse anti-HPV 16 L1 ab a while back. I am using the ab for ELISA purposes (to test levels of HPV specific IgG). I am trying to figure out appropriate standards for my ELISA. At first, I was thinking that I might coat a plate with anti-mouse IgG, titrate purified normal mouse IgG, and probe with a goat anti-mouse IgG-HRP Ab. But I wonder, if I coat with anti-mouse IgG, could I titrate with the Ab69 and pick up a signal after probing with goat anti-mouse IgG-HRP. I'm thinking the answer is no, but wanted to check. Could you help me figure out appropriate standards for using this antibody in ELISA format?

    Read More
    Answer

    Thank you for contacting us.


    If I understand you correctly. The anti-mouse IgG would recognize your purified normal mouse IgG or it would recognize Ab69. These, in turn, would be recognized by goat anti-mouse HRP. However if you are to use ab69 against HPV in mouse tissues your detection antibody (goat anti-mouse IgG-HRP) should IgG2a specific,as it may recognize both ab69 and the Fc receptors in the tissue thereby creating a false positive.

    It seems that youare proposing a sandwich ELISA. These can be difficult to optimize. The antibodies must be detecting different epitopes of the antigen so they do not inferfere with the other antibody binding. We are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested in that application.

    If ab69 is your detection


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    To Whom It May Concern:   I am requesting an MSDS (Material Safety Data Sheet) for a product used by a client of mine, Thunder Bay Regional Research.  The products are as follows:   -HPV16 L1 (product code: ab69) -HPV16 E2 (product code: ab17185) -mouse monoclonal to CD11C (BU15) (product code: ab11029) -Carbonic anhydrase 9 (product code: AB47011) -ChIP Kit (product code: AB500) -mouse monoclonal to HPV16 E6 and HPV 18 E6 (C1P5) (product code: ab70)   Are these products exempt from having an msds?  

    Read More
    Answer

    Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me know.

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    Question

    Dear technical team,   Our customer would like to know more information about immunogen of ab69 and ab30908.   If you have information of amino acid sequence, please let me know.   Thank you in advance.

    Read More
    Answer

    Thank you for your inquiry. Both Anti-HPV16 L1 recognise HPV Type 16, L1 (major capsid protein). Unfortunately, we do not currently have more information about the epitopes for those monoclonal antibodies. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

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    Question

    We routinely buy antibodies from Abcam, so I was wondering if you could send us a small amount of the ab69 HPV16-L1 (CamVir 1) antibody to test before we purchase a larger amount.

    Read More
    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 100 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.

    Read More

    1-10 of 13 Abreviews or Q&A

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