Anti-HPV16 E6 + HPV18 E6 抗体 [C1P5] (ab70)


  • 製品名
    Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]
    HPV16 E6 + HPV18 E6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6
  • 由来種
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 免疫原

    Gel-purified HPV-18 E6-beta galactosidase fusion protein.

  • ポジティブ・コントロール
    • Human cervical cancer cells.
  • 特記事項

    Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

    The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.



Our Abpromise guarantee covers the use of ab70 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.

16.5 kDa band is HPV-18, 17 kDa band is HPV-16.

The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • 関連性
    Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
  • 別名
    • E6 antibody
    • Human Papilloma Virus antibody
    • Human papillomavirus type 16 E6 antibody
    • Human papillomavirus type 18 E6 antibody
    • Protein E6 antibody
    see all


  • Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.


This product has been referenced in:
  • Wang X  et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28480962) »
  • Mitkevich VA  et al. Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins. Oncotarget 8:72666-72675 (2017). Read more (PubMed: 29069817) »

See all 16 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Uterine cervix, Squamous cell carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0
Uterine cervix, Squamous cell carcinoma

Mr. Allan Wang

Verified customer

投稿 Apr 17 2018

Thank you for your inquiry

I heard back from the lab that, unfortunately, they do not have any information relating to the structure of the antibody. But this is a standard antibody so I would expect that is does have an Fc region.


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Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

Here is the discoun...

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Thank you for contacting us and your interest in our products.

The antibodies ab70 and ab30716 are in fact the same antibody, produced from different sources.I can understand that this is currently not reflected very well on our website but w...

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Abcam has not validated the combination of species/application used in this Abreview.
Cow Cultured Cells (Epithelial cells of cows with BPV and cells free-B)
Epithelial cells of cows with BPV and cells free-B
Yes - Tritonx100 (0,1%),
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Aug 31 2012

Thank you for your reply.

I have located the Abreview in our system for ab70. It seems that the customer has begun this review, but must provide the product lot number (found on the vial) in order to officially submit the review. Therefor...

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Thank you for the update and I am really happy that the customer was satisfied with the outcome.

If you have any other situations like this or if there is anything else we can help with, then please let me know.

Thank you very much for contacting us a while back about the HPV16E6 + HPV18 E6 antibody ab70.

I sincerely apologize for the extended delay in getting this information for you, and I appreciate your patience while I have been in touch with th...

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Thank you for your reply.

As we have not previously tested this antibody in ICC/IF, we could not guarantee that it would work for this application, as under our Abpromise we only guarantee it to work in WB, IP& IHC-P.

However, I ...

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Thank you very much for your interest in (Anti-HPV16 E6 + HPV18 E6 antibody [C1P5])

To our knowledge,ab70 has not been tested with p16ink4a. Therefore, I can offer a discount off a future purchase if you buy ab70 now...

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1-10 of 22 Abreviews or Q&A