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Anti-HPV11 E7 抗体 [9H5] (ab100967)

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Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)
  • Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)

Key features and details

  • Mouse monoclonal [9H5] to HPV11 E7
  • Suitable for: WB, IP, ELISA, ICC/IF
  • Reacts with: Human papillomavirus
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-HPV11 E7 antibody [9H5]

  • 製品の詳細

    Mouse monoclonal [9H5] to HPV11 E7

  • 由来種

    Mouse

  • 特異性

    Reacts in WB, IP, ELISA with HPV11 native E7 protein as well as recombinant E7 protein expressed in bacteria and Cos7 cells.

  • アプリケーション

    適用あり: WB, IP, ELISA, ICC/IFmore details

  • 種交差性

    交差種: Human papillomavirus

  • 免疫原

    Recombinant full length HPV11 E7 protein purified from bacteria.

  • エピトープ

    Mapped to amino acids 36-70

  • ポジティブ･コントロール

    • WB: U2OS and HeLa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV-11 E7 protein IP: lysate of U20S cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    9H5

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab100967の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 11 kDa.
IP
1/500.
ELISA
1/128000.
ICC/IF
Use at an assay dependent dilution.
特記事項
WB
1/1000. Predicted molecular weight: 11 kDa.
IP
1/500.
ELISA
1/128000.
ICC/IF
Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    Infections by human papillomaviruses (HPV) can cause relatively harmless plantar warts and also more serious genital warts that can increase the risk to women for developing cervical cancer. Infections with HPV11 triggers genital warts. E7 is a protein that is encoded by by HPV11 and is implicated in the disruption of host cells and promoting their proliferation.

  • 参照データベース

    • 別名

      • Human papillomavirus E7 protein antibody

    画像

    • Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)
      Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)
      All lanes : Anti-HPV11 E7 antibody [9H5] (ab100967) at 1/1000 dilution

      Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
      Lane 2 : lysate of plain U20S cells
      Lane 3 : lysate of HeLa cells
      Lane 5 : marker

      Predicted band size: 11 kDa

    • Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)
      Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)
      Immunoprecipitation (IP) analysis of HPV11 E7 protein.
      Lane 1 represents the marker.
      Lane 2 shows detection of E7 protein by ab100967 in lysate of U20S cells.
      In lane 3 the lower band is precipitated E7, upper bands are light and heavy chain of the antibody.
      Lane 4 is precipitation without ab100967
      Lane 5 shows IP experiment with plain U2OS cells.
    • Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)
      Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)
      Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells.
      IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7 detected by ab100967. IF analysis of E7 protein shows similar result also in HeLa cells.

    参考文献 (0)

    ab100967 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab100967 は論文での使用が確認できていません。

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