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Anti-HPV11 E2 抗体 [10E12] (ab100968)

Submit a review Submit a question References (2)
Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (ab100968)

Key features and details

  • Mouse monoclonal [10E12] to HPV11 E2
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Human papillomavirus
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HPV11 E2 antibody [10E12]

  • 製品の詳細

    Mouse monoclonal [10E12] to HPV11 E2

  • 由来種

    Mouse

  • 特異性

    Reacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.

  • アプリケーション

    適用あり: WB, ELISA, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human papillomavirus

  • 免疫原

    Recombinant full length HPV11 E2 protein purified from bacteria.

  • エピトープ

    Mapped to amino acids 202-284

  • ポジティブ･コントロール

    • U20S or Hela cells and lysates

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified from bacteria by affinity chromatography.

  • ポリ/モノ

    モノクローナル

  • クローン名

    10E12

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab100968の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 42 kDa.
ELISA
1/32000.
ICC/IF
Use at an assay dependent dilution.
特記事項
WB
1/1000. Predicted molecular weight: 42 kDa.
ELISA
1/32000.
ICC/IF
Use at an assay dependent dilution.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 関連性

    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.

  • 細胞内局在

    Host nucleus

  • 参照データベース

    • 別名

      • Human papillomaviruses 11 E2 antibody

    画像

    • Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968)
      Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968)
      All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution

      Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing
      HPV11 E2 protein
      Lane 2 : lysate of plain U20S cells
      Lane 3 : lysate of plain HeLa cells
      Lane 5 : marker

      Predicted band size: 42 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (ab100968)
      Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (ab100968)
      Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
      IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.

    参考文献 (2)

    ab100968 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab100968 は 2 報の論文で使用されています。

    • Nanbo A  et al. Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse. J Gen Virol 97:2989-3006 (2016). PubMed: 27655016
    • Kivipõld P  et al. DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells. Virol J 12:104 (2015). IF . PubMed: 26148509

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