Anti-HPV 抗体 [BPV-1/1H8 + CAMVIR] (ab2417)

Reviews (1)Q&A (8)References (11)

製品の概要

  • 製品名

    Anti-HPV antibody [BPV-1/1H8 + CAMVIR]
    HPV 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV

  • 由来種

    Mouse

  • 特異性

    The BPV-1/1H8 clone recognizes HPV-1, 6, 11 and 16. The CAMVIR clone recognizes HPV-16, 18 and 31.

    Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.

  • アプリケーション

    適用あり: IHC-P, ELISA, WBmore details

  • 種交差性

    交差種: Human papillomavirus

  • 免疫原

    This product was produced with the following immunogens:
    Recombinant full length protein. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein.

    Tissue, cells or virus. The BPV-1/1H8 antibody was raised against SDS-disrupted bovine papillomavirus type 1(BPV-1)

  • ポジティブ･コントロール

    • IHC-P: Infected cervical biopsy tissue. WB: Control and DCT siRNA transfected HaCaT cell lysate.

  • 特記事項

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is a mix of BPV-1/1H8 (IgG) and CAMVIR (IgG2a).

    The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent concentration. PubMed: 22479413

ターゲット情報

  • 関連性

    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer.

  • 細胞内局在

    Nuclear

  • 別名

    • HPV antibody
    • Human papillomavirus antibody

画像

  • Western blot - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)
    Western blot - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)Aksoy P et al PLoS One. 2017 Jan 17;12(1):e0170158. doi: 10.1371. Figure 1 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot analysis of L1 and actin levels in (A) uninfected, control and DCT siRNA transfected HaCaTs.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)
    ab2417 staining HPV-infected human cervix tissue sections.

参考文献

This product has been referenced in:

  • Lange CE  et al. Canine papillomavirus types 1 and 2 in classical papillomas: High abundance, different morphological associations and frequent co-infections. Vet J 250:1-5 (2019). Read more (PubMed: 31383413) »
  • Larmour LI  et al. A patient derived xenograft model of cervical cancer and cervical dysplasia. PLoS One 13:e0206539 (2018). Read more (PubMed: 30365542) »
See all 11 Publications for this product

ab2417 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-9 of 9 Abreviews or Q&A

Question


You could recommend me a secondary antibody for WB and ELISA against
monoclonal mouse [BPV-1/1H8 + CAMVIR]ab2417, please.

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Answer

I recommend ab97023, goat anti-mouse IgG H&L (heavy and light chains), assuming you want an HRP conjugate (horseradish peroxidase) for visualization. We also have fluorochrome conjugates and alkaline phosphatase conjugates, among others.

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Western blot abreview for Anti-HPV antibody [BPV-1/1H8 + CAMVIR]

 Poor
Abreviews
Application
Western blot
Sample
Tobacco Recombinant protein (Plant cell)
Loading amount
21 µg
Specification
Plant cell
Gel Running Conditions
Reduced Denaturing (gel 12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Mr. Diego Grando Módolo

Verified customer

投稿 Dec 18 2012

Question

The formulation buffer for this product includes "Tissue Culture Supernatant". What are the constituents of this supernatant? Is 10% FBS or simiar included?

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Answer

Thank you for contacting Abcam

Theprotein carrier forHPV Cocktail Broad Spectrumis heat attenuated goat serum.

Please let me know if there is anything else I can help you with.

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Question

Hi,

I'm interested inantibody "ab30908".

And I want to knowthis antibody can cross react withL1 protein of another type of HPV.

If you don't have any information about cross reactivity,can you give mesome information about the sequence of immunogen?

And I also want to know "ab2417" and "ab75574" can react withL1 protein of HPV.

Can I use these antibodies in WB?

Regards,

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Answer

Thank you for your enquiry and for your patience. It has taken some time to gather the information.

Regardnig ab2417 Anti-HPV antibody [BPV-1/1H8 + CAMVIR], I can confirm thiswas raised against the major capsid protein L1. I have copied below some further information:

The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

Regarding ab30908HPV16 L1 antibody [289-16981], this should detectL1 as it was raised against HPV Type 16L1 protein.

The ab75574 Anti-HPV antibody [K1H8],hasbeen sourced and tested externally. I am sorry we have not been able to obtain any further information yet for these antibodies, and therefore I would assume this has regrettably not been specifically tested for detection of L1.

For western blotting, I can confirm that all tested applications covered by our guarantee are listed on the online datasheets which are updated as soon as we receive further information. I can confirm that ab2417 and ab30908 have been tested in WB. The ab75574 has not been tested in WB.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Do you have a MSDS sheet for MsmAb to HPV(BPV-1-1H8)ab2417-250abcam?

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Answer

Thank you for contacting us.

My colleague has created an SDS for ab2417 and it is now available for download from our website. I have also attached it to this email. Please let me know if you need any additional information.

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Question

I am getting some staining in cytoplasm and no staining in nuclear region.

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Answer

Thanks you for your enquiry. Based on the following publications, there are cases where the HPV expression was found in the cytoplasm and nuclear region. 1) CASE REPORT: Corneal papilloma associated with papillomavirus in a one-humped camel (Camelus dromedarius). Veterinary Ophthalmology. Volume 13, Issue Supplement s1, pages 100–102, September 2010. (Fig 3) http://onlinelibrary.wiley.com/doi/10.1111/j.1463-5224.2010.00795.x/full 2) Clinical, pathological and immunohistochemical findings of bovine　cutaneous papillomatosis. Ankara Üniv Vet Fak Derg, 58, 161-165, 2011 (Fig 2) http://dergiler.ankara.edu.tr/dergiler/11/1565/16994.pdf Therefore, the results you are seeing is not uncommon. Although on our datasheet it does state nuclear, this is information from SwissProt and may not be the case in an actual experiment. I hope this information will be helpful.

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Question

Anti-HPV antibody [BPV-1-1H8] (ab2417) I note from the web page [https://www.abcam.com/HPV-antibody-BPV-1-1H8-ab2417.html]that it states this is also an anti E6/early transforming protein E6 antibody, under the Alternative Names section. This cannot be true if it has been raised against whole (BPV-1) virus, because E6 is made only after infection and is not packaged within the virus. Anti-BPV L1 would be an alternative name. It would be worrying for someone to confuse E6 and L1 in either research or diagnostic uses of this antibody, so it might be worth correcting the mistake. The ELISA also confirms its reactivity with purified L1 (not E6).  Unless I am horribly confused.  We shall shortly be ordering this antibody to test it in various non-human species  Cheers!      

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Answer

Thank you for your enquiry regarding ab2417. After discussing your comments with some experts, we have reviewed the alternative names on the datasheet and amended it. We apologize for any inconvenience caused and thank you for bringing this matter to our attention. If you need any further assistance in the future, please do not hesitate to contact me.  

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Question

which dilution you suggest for IHC , paraffin sections for this antibody ? Thanks and regards

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Answer

Thank you for your enquiry. This antibody was used at a 1:80 dilution and the secondary antibody was a MACH3 Mouse AP Polymer. Please bear in mind that different samples and buffers used may require some optimization. I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!

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Question

I'm writing to you for asking you a question and that is I'm interested in knowing an exact diluition for immunofluorescence for the antibody HPV (BPV-1-1H8) cod ab2417 that we're interested in ordering!

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Answer

The antibody was used at a concentration of 1:80 in IHC-P. Secondary was AP-polymer. We have not carried out ICC/IF for this antibody. Thus, we are unable to guarantee the suitability of this application. Probably antibody could be further diluted.

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