Anti-HPV 抗体 [BPV-1/1H8 + CAMVIR] (ab2417)
Key features and details
- Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV
- Suitable for: IHC-P, ELISA
- Reacts with: Human papillomavirus
- Isotype: IgG
製品の概要
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製品名
Anti-HPV antibody [BPV-1/1H8 + CAMVIR]
HPV 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV -
由来種
Mouse -
特異性
The BPV-1/1H8 clone recognizes HPV-1, 6, 11 and 16. The CAMVIR clone recognizes HPV-16, 18 and 31.
Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.
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アプリケーション
適用あり: IHC-P, ELISAmore details -
種交差性
交差種: Human papillomavirus -
免疫原
This product was produced with the following immunogens:
Recombinant full length protein. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein.
Tissue, cells or virus. The BPV-1/1H8 antibody was raised against SDS-disrupted bovine papillomavirus type 1(BPV-1) -
ポジティブ・コントロール
- IHC-P: Infected cervical biopsy tissue. WB: Control and DCT siRNA transfected HaCaT cell lysate.
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特記事項
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is a mix of BPV-1/1H8 (IgG) and CAMVIR (IgG2a).
The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
BPV-1/1H8 + CAMVIR -
ミエローマ
unknown -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2417の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. -
細胞内局在
Nuclear -
別名
- HPV antibody
- Human papillomavirus antibody
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab2417 は 14 報の論文で使用されています。
- Chang CY et al. The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs. Viruses 12:N/A (2020). PubMed: 32028559
- Lange CE et al. Canine papillomavirus types 1 and 2 in classical papillomas: High abundance, different morphological associations and frequent co-infections. Vet J 250:1-5 (2019). PubMed: 31383413
- Gang DG et al. Sebaceous cell differentiation in a canine oral papilloma. J Vet Diagn Invest 30:569-571 (2018). PubMed: 29860939
- Larmour LI et al. A patient derived xenograft model of cervical cancer and cervical dysplasia. PLoS One 13:e0206539 (2018). PubMed: 30365542
- Aksoy P & Meneses PI The Role of DCT in HPV16 Infection of HaCaTs. PLoS One 12:e0170158 (2017). WB . PubMed: 28095444