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  1. Link

    hprt-antibody-ab10479.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA / Nucleotides
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遺伝子ノックアウト細胞株 検証済

Anti-HPRT 抗体 (ab10479)

  • Datasheet
Reviews (4) Submit a question References (45)

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Western blot - Anti-HPRT antibody (ab10479)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPRT antibody (ab10479)
  • Western blot - Anti-HPRT antibody (ab10479)
  • Western blot - Anti-HPRT antibody (ab10479)
  • Western blot - Anti-HPRT antibody (ab10479)

Key features and details

  • Rabbit polyclonal to HPRT
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-HPRT antibody [EPR5298] (ab133242)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-HPRT antibody
    HPRT 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to HPRT
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Gerbil, Chinese hamster
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • Recombinant Human HPRT protein (ab117153) can be used as a positive control in WB. This antibody gave a positive control in the following human whole cell lysates: HeLa, A431, MCF-7, HEK 293 whole cell lysate This antibody gave a positive control in the following mouse lysates: NIH 3T3, MEF1 whole cell lysate, mouse brain tissue lysate
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Cell Biology
    • Other Antibodies
    • Other Antibodies

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HPRT protein (Active) (ab222951)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab10479の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
1/500 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
  • パスウェイ

    Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.
  • 関連疾患

    Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.
    Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT) [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia.
  • 配列類似性

    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P00492 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 395653 Chicken
    • Entrez Gene: 3251 Human
    • Entrez Gene: 15452 Mouse
    • Entrez Gene: 24465 Rat
    • Omim: 308000 Human
    • SwissProt: Q9W719 Chicken
    • SwissProt: P00492 Human
    • SwissProt: P00493 Mouse
    • SwissProt: P27605 Rat
    • Unigene: 412707 Human
    • Unigene: 299381 Mouse
    • Unigene: 47 Rat
    see all
  • 別名

    • HGPRT antibody
    • HGPRTase antibody
    • HPRT 1 antibody
    • HPRT_HUMAN antibody
    • HPRT1 antibody
    • Hypoxanthine guanine phosphoribosyltransferase antibody
    • Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody
    • Hypoxanthine phosphoribosyltransferase 1 antibody
    • Hypoxanthine-guanine phosphoribosyltransferase antibody
    see all

画像

  • Western blot - Anti-HPRT antibody (ab10479)
    Western blot - Anti-HPRT antibody (ab10479)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A431 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab10479 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab10479 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were examined. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE.  Ab10479 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-HPRT antibody (ab10479)
    Immunocytochemistry/ Immunofluorescence - Anti-HPRT antibody (ab10479)

    ICC/IF image of ab10479 stained human HeLa cells. The cells were methanol fixed ( 5 min) and incubated with the antibody (ab10479, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

  • Western blot - Anti-HPRT antibody (ab10479)
    Western blot - Anti-HPRT antibody (ab10479)
    All lanes : Anti-HPRT antibody (ab10479) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A-431 whole cell lysate (ab7909)
    Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK-293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa

  • Western blot - Anti-HPRT antibody (ab10479)
    Western blot - Anti-HPRT antibody (ab10479)
    All lanes : Anti-HPRT antibody (ab10479) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa
    Additional bands at: 28 kDa (possible glycosylated form)

  • Western blot - Anti-HPRT antibody (ab10479)
    Western blot - Anti-HPRT antibody (ab10479)

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (45)

ab10479 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab10479 は 45 報の論文で使用されています。

  • Xu X  et al. Rewiring of purine metabolism in response to acidosis stress in glioma stem cells. Cell Death Dis 12:277 (2021). PubMed: 33723244
  • Chaiyawat P  et al. IMPDH2 and HPRT expression and a prognostic significance in preoperative and postoperative patients with osteosarcoma. Sci Rep 11:10887 (2021). PubMed: 34035425
  • Thomas JD  et al. RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons. Nat Genet 52:84-94 (2020). PubMed: 31911676
  • Townsend MH  et al. Evaluation of the upregulation and surface expression of hypoxanthine guanine phosphoribosyltransferase in acute lymphoblastic leukemia and Burkitt's B cell lymphoma. Cancer Cell Int 20:375 (2020). PubMed: 32782434
  • Chan DY  et al. Multigene human artificial chromosome vector delivery with herpes simplex virus 1 amplicons. Exp Cell Res 388:111840 (2020). PubMed: 31930965
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-HPRT antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP1 Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
THP1 Cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

投稿 Jul 27 2018

Western blot

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Sample
Mouse Cell lysate - whole cell (beta cells)
Specification
beta cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jun 05 2013

Western blot abreview for Anti-HPRT antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
5 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Sep 02 2010

Western blot abreview for Anti-HPRT antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse striatum and liver)
Loading amount
20 µg
Specification
Mouse striatum and liver
Gel Running Conditions
Reduced Denaturing (4-12% TG)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Dr. Laura Ling

Verified customer

投稿 Jul 31 2008

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