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  1. Link

    hp1-gammacbx3-antibody-ab10480.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methylated DNA
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遺伝子ノックアウト細胞株 検証済

Anti-HP1 gamma/CBX3 抗体 (ab10480)

  • Datasheet
Reviews (9)Q&A (2)References (22)

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Abpromise

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Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
  • Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
  • Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)

Key features and details

  • Rabbit polyclonal to HP1 gamma/CBX3
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-HP1 gamma/CBX3 antibody
    HP1 gamma/CBX3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to HP1 gamma/CBX3
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; MCF7; HEK293.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methylated DNA
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human HP1 gamma/CBX3 protein (ab124548)
  • Recombinant Protein

    • Recombinant Human HP1 gamma/CBX3 protein (ab124548)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab10480の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
特記事項
WB
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).

ターゲット情報

  • 機能

    Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • 配列類似性

    Contains 2 chromo domains.
  • 翻訳後修飾

    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • 細胞内局在

    Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Target information above from: UniProt accession Q13185 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 11335 Human
    • Entrez Gene: 12417 Mouse
    • Omim: 604477 Human
    • SwissProt: Q13185 Human
    • SwissProt: P23198 Mouse
    • Unigene: 381189 Human
    • Unigene: 280968 Mouse
    • Unigene: 458234 Mouse
    • Unigene: 485141 Mouse
    see all
  • 別名

    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

画像

  • Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
    Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab10480 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab10480 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab10480 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
    Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
    All lanes : Anti-HP1 gamma/CBX3 antibody (ab10480) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?
    Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
    Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
    Anti-HP1 gamma/CBX3 antibody (ab10480) at 1/500 dilution + Recombinant Human HP1 gamma protein (ab88011) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa


    Exposure time: 30 seconds


    Ab10480 recognizes the tagged recombinant HP1 gamma/CBX3 protein (ab88011) which has an expected molecular weight of ~50 kDa.

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (22)

ab10480 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab10480 は 22 報の論文で使用されています。

  • Ma C  et al. CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma. Mol Med Rep 19:4205-4212 (2019). PubMed: 30942427
  • Feiner B  et al. Risperidone effects on heterochromatin: the role of kinase signaling. Clin Exp Immunol 196:67-75 (2019). PubMed: 30714144
  • Zhao SP  et al. CBX3 promotes glioma U87 cell proliferation and predicts an unfavorable prognosis. J Neurooncol 145:35-48 (2019). PubMed: 31502042
  • Gomes AP  et al. Dynamic Incorporation of Histone H3 Variants into Chromatin Is Essential for Acquisition of Aggressive Traits and Metastatic Colonization. Cancer Cell 36:402-417.e13 (2019). PubMed: 31564638
  • Poulard C  et al. Increasing G9a automethylation sensitizes B acute lymphoblastic leukemia cells to glucocorticoid-induced death. Cell Death Dis 9:1038 (2018). PubMed: 30305606
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 11 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-HP1 gamma/CBX3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HT-22 (neuronal cell line))
Permeabilization
No
Specification
HT-22 (neuronal cell line)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Dec 30 2021

Immunohistochemistry (Frozen sections) abreview for Anti-HP1 gamma/CBX3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain cortex)
Permeabilization
No
Specification
Brain cortex
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Dec 30 2021

Western blot abreview for Anti-HP1 gamma/CBX3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (293T)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
15 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Dec 16 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HP1 gamma/CBX3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse 14.5dpc)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9
Permeabilization
No
Specification
Mouse 14.5dpc
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 15 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HP1 gamma/CBX3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin, epidermis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
Yes - TBST
Specification
Skin, epidermis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 16 2010

Immunocytochemistry abreview for Anti-HP1 gamma/CBX3 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Colorectal Cancer Cells)
Permeabilization
Yes - Triton X-100
Specification
Colorectal Cancer Cells
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 06 2010

Western blot abreview for Anti-HP1 gamma/CBX3 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Colorectal Cancer Cells)
Gel Running Conditions
Reduced Denaturing (12% Gel)
Loading amount
200 µg
Specification
Colorectal Cancer Cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Abcam user community

Verified customer

投稿 Jan 04 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HP1 gamma/CBX3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Colon Cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO Target Retrieval Solution
Permeabilization
No
Specification
Human Colon Cancer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 03 2008

Question

- The antibody fials in chip
- other 10 antibodies worked well
- Optimized the antibodies but no success

Read More

Abcam community

Verified customer

Asked on Jan 09 2013

Answer

Thank you for contacting us.

I am attaching few questions here, please answers these as these will help us to investigate what went wrong with these antibodies;

Sample preparation (X-ChIP/N-ChIP, cross-linking conditions, DNA fragment size)
Immunoprecipitation conditions (buffer, amount of detergent, primary antibody manufacturer/dilution, incubation time, matrix for isolating antibody complex)
Wash steps
DNA purification (decrosslinking conditions, DNA purification method)
DNA detection method (regions amplified in PCR, microarray, direct sequencing
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 09 2013

Question

Wish to try ab10480 on mouse samples.

Read More

Abcam community

Verified customer

Asked on May 08 2012

Answer

DISCOUNT CODE: ********
Expiration date: ********

I am very pleased to hear you would like to accept our offer and test ab10480in mouse tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on May 08 2012

1-10 of 11 Abreviews or Q&A

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