Anti-HP1 alpha 抗体 [3A11F8] (ab234085)
Key features and details
- Mouse monoclonal [3A11F8] to HP1 alpha
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-HP1 alpha antibody [3A11F8]
HP1 alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3A11F8] to HP1 alpha -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human HP1 alpha aa 1 to the C-terminus. Expressed in E. coli.
Database link: P45973 -
ポジティブ・コントロール
- WB: HeLa, NIH/3T3, K562, MCF7, Jurkat and A431 cell lysate; Recombinant human HP1 alpha (amino acids: 1-191) protein; HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow Cytometry: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
3A11F8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab234085の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100 - 1/500. Predicted molecular weight: 22 kDa.
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Flow Cyt |
1/200 - 1/400.
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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WB
1/100 - 1/500. Predicted molecular weight: 22 kDa. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
配列類似性
Contains 2 chromo domains. -
翻訳後修飾
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
- Unigene: 262059 Mouse
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別名
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
画像
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All lanes : Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 6 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 22 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 alpha (green) using ab234085 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor®555-Phalloidin.
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Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling HP1 alpha with ab234085 at 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 22 kDa -
Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution + Recombinant human HP1 alpha (amino acids: 1-191) protein
Predicted band size: 22 kDaExpected recombinant MW is 48.2 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab234085 は論文での使用が確認できていません。