Anti-HOXD13 抗体 (ab19866)
Key features and details
- Rabbit polyclonal to HOXD13
- Suitable for: WB, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-HOXD13 antibody
HOXD13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HOXD13 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-FoFr, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Xenopus laevis
-
免疫原
Synthetic peptide corresponding to Human HOXD13 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab22466) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19866の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 36 kDa).
|
|
| IHC-FoFr |
1/100 - 1/300.
|
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 36 kDa). |
|
IHC-FoFr
1/100 - 1/300. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
関連性
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 396415 Chicken
- Entrez Gene: 3239 Human
- Entrez Gene: 15433 Mouse
- Entrez Gene: 288154 Rat
- Omim: 142989 Human
- SwissProt: P24344 Chicken
- SwissProt: P35453 Human
- SwissProt: P70217 Mouse
-
別名
- HOXD13 antibody
- BDE antibody
- BDSD antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-HOXD13 antibody (ab19866)
ICC/IF image of ab19866 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab19866 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-HOXD13 antibody (ab19866)This image is courtesy of Shunyou Wang, Ph.D.ab19866 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) lung. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG. -
Western blot - Anti-HOXD13 antibody (ab19866)Anti-HOXD13 antibody (ab19866) at 1 µg/ml + HOXD13 (Human) Recombinant Protein at 0.2 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?ab19866 was tested on a recombinant GST tagged HOXD13 protein (Human) predicted to run at 65 kDa.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (8)
ab19866 は 8 報の論文で使用されています。
- Bolt CC et al. Mesomelic dysplasias associated with the HOXD locus are caused by regulatory reallocations. Nat Commun 12:5013 (2021). PubMed: 34408147
- Basu S et al. Unblending of Transcriptional Condensates in Human Repeat Expansion Disease. Cell 181:1062-1079.e30 (2020). PubMed: 32386547
- Tatangelo F et al. Posterior HOX genes and HOTAIR expression in the proximal and distal colon cancer pathogenesis. J Transl Med 16:350 (2018). PubMed: 30541551
- Aquino G et al. Deregulation of paralogous 13 HOX genes in oral squamous cell carcinoma. Am J Cancer Res 5:3042-55 (2015). PubMed: 26693058
- Cantile M et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . PubMed: 24189220
- Illig R et al. Spatio-temporal expression of HOX genes in human hindgut development. Dev Dyn 242:53-66 (2013). PubMed: 23073994
- Illig R et al. Breaking the seals: efficient mRNA detection from human archival paraffin-embedded tissue. RNA 15:1588-96 (2009). IHC-P ; Human . PubMed: 19549718
- Cantile M et al. HOX D13 expression across 79 tumor tissue types. Int J Cancer 125:1532-41 (2009). PubMed: 19488988
