Anti-HOXB5 抗体 [EP2827] (ab109375)
![Western blot - Anti-HOXB5 antibody [EP2827] (ab109375)](https://www.abcam.co.jp/ps/products/109/ab109375/Images/ab109375-122155-anti-hoxb5-antibody-ep2827-western-blot.jpg "Western blot - Anti-HOXB5 antibody [EP2827] (ab109375)")
Key features and details
- Rabbit monoclonal [EP2827] to HOXB5
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-HOXB5 antibody [EP2827]
HOXB5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2827] to HOXB5 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal lung and Human fetal kidney lysates
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2827 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109375の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
ターゲット情報
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機能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
組織特異性
Spinal cord. -
配列類似性
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain. -
発生段階
Embryo. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3215 Human
- Entrez Gene: 15413 Mouse
- Entrez Gene: 497987 Rat
- Omim: 142960 Human
- SwissProt: P09067 Human
- SwissProt: P09079 Mouse
- Unigene: 654456 Human
- Unigene: 207 Mouse
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別名
- HGNC:5116 antibody
- HHO.C10 antibody
- Homeo box 2A antibody
see all
画像
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Western blot - Anti-HOXB5 antibody [EP2827] (ab109375)All lanes : Anti-HOXB5 antibody [EP2827] (ab109375) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 29 kDa -
![Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] (ab109375)](https://www.abcam.co.jp/ps/products/109/ab109375/Images/ab109375-333873-anti-hoxb5-antibody-ep2827-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] (ab109375)ab109375 staining the HOXB5 in U-87 MG (Human glioblastoma-astrocytoma epithelial cell) by intracellular flow cytometry. The sample was incubated with the primary antibody at 1/80 (Pink) for 30 minutes at 4°C. A FITC-conjugated Goat anti-rabbit IgG (1/150) was used as the secondary antibody.
Rabbit monoclonal IgG was used as an Isotype control (Green)
データシートおよび資料
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Datasheet download
参考文献 (4)
ab109375 は 4 報の論文で使用されています。
- Zhao J et al. The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway. J Exp Clin Cancer Res 40:134 (2021). PubMed: 33858489
- Chen M et al. The Prognostic Value and Function of HOXB5 in Acute Myeloid Leukemia. Front Genet 12:678368 (2021). PubMed: 34421991
- Lin C et al. Long non-coding RNA PRNCR1 exerts oncogenic effects in tongue squamous cell carcinoma in vitro and in vivo by sponging microRNA-944 and thereby increasing HOXB5 expression. Int J Mol Med 46:119-130 (2020). PubMed: 32319550
- Wang Y et al. CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway. J Exp Clin Cancer Res 39:169 (2020). PubMed: 32847606
