Horse VEGFA ELISA kit (ab272034)

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Horse VEGFA standard curve

    Key features and details

    • Sensitivity: 4 pg/ml
    • Range: 4.1 pg/ml - 1000 pg/ml
    • Sample type: Cell culture media, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Horse

    製品の概要

    • 製品名

      Horse VEGFA ELISA kit
      VEGFA キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall < 10%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall < 12%

    • サンプルの種類

      Serum, Plasma, Cell culture media

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      4 pg/ml

    • 検出範囲

      4.1 pg/ml - 1000 pg/ml

    • 添加回収試験

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Serum 105.3 101% - 110%
      Plasma 113.2 103% - 125%
      Cell culture media 123 115% - 133%

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Horse

    • 製品の概要

      Horse VEGFA ELISA Kit is designed for the quantitative determination of Horse VEGFA in cell culture supernatants, plasma and serum samples.


      This assay employs an antibody specific for VEGFA coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGFA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse VEGFA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 試験プラットフォーム

      Pre-coated microplate (12 x 8 well strips)

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      500X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent B 1 x 15ml
      5X Assay Diluent D 1 x 15ml
      Anti-Horse VEGFA coated Microplate (12 x 8 wells) 1 unit
      Biotinylated Anti-Horse VEGFA Detection Antibody 2 vials
      Horse VEGFA Standard (Lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB Substrate Solution 1 x 12ml

    • 研究分野

    • 機能

      Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

    • 組織特異性

      Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

    • 関連疾患

      Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

    • 配列類似性

      Belongs to the PDGF/VEGF growth factor family.

    • 細胞内局在

      Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
    • Information by UniProt

    • 別名

      • Folliculostellate cell-derived growth factor
      • Glioma-derived endothelial cell mitogen
      • MGC70609
      • MVCD1
      • vascular endothelial growth factor
      • Vascular endothelial growth factor A
      • vascular endothelial growth factor A121
      • vascular endothelial growth factor A165
      • Vascular permeability factor
      • Vegf
      • VEGF A
      • VEGF-A
      • VEGF120
      • Vegfa
      • VEGFA_HUMAN
      • VPF
      see all

    関連製品

      アプリケーション

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab272034 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      アプリケーション Abreviews 特記事項
      Sandwich ELISA
      Use at an assay dependent concentration.
      特記事項
      Sandwich ELISA
      Use at an assay dependent concentration.

      画像

      • Horse VEGFA standard curve
        Horse VEGFA standard curve

        This standard curve is for demonstration only. A standard curve must be run with each assay.

        Horse VEGF-A ELISA kit (ab272034) Standard curve.

      参考文献 (0)

      ab272034 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272034 は論文での使用が確認できていません。

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