Horse TIMP2 ELISA kit (ab272033)

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Horse TIMP-2 Standard Curve

    Key features and details

    • Sensitivity: 4 pg/ml
    • Range: 16.38 pg/ml - 4000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Horse, Human

    製品の概要

    • 製品名

      Horse TIMP2 ELISA kit
      TIMP2 キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall < 10%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall < 12%

    • サンプルの種類

      Cell culture supernatant, Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      4 pg/ml

    • 検出範囲

      16.38 pg/ml - 4000 pg/ml

    • 添加回収試験

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Cell culture supernatant 141.3 131% - 149%
      Serum 141.2 135% - 148%
      Plasma 143.3 137% - 149%

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Horse, Human

    • 製品の概要

      Horse TIMP-2 ELISA Kit is designed for the quantitative determination of Horse TIMP-2 in cell culture supernatants, plasma and serum samples.


      This assay employs an antibody specific for TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      Detects horse and human TIMP2.

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 試験プラットフォーム

      Pre-coated microplate (12 x 8 well strips)

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab272033 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Horse TIMP-2 Standard Curve
      Horse TIMP-2 Standard Curve

      This standard curve is for demonstration only. A standard curve must be run with each assay.

      Horse TIMP-2 ELISA kit (ab272033) Standard curve.

    参考文献 (0)

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