Horse IP-10 ELISA kit (CXCL10) (ab272036)

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Horse IP-10 standard curve

    Key features and details

    • Sensitivity: 7 pg/ml
    • Range: 6.4 pg/ml - 1500 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Horse

    製品の概要

    • 製品名

      Horse IP-10 ELISA kit (CXCL10)
      IP10 キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall < 10%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall < 12%

    • サンプルの種類

      Cell culture supernatant, Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      7 pg/ml

    • 検出範囲

      6.4 pg/ml - 1500 pg/ml

    • 添加回収試験

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Cell culture supernatant 139.6 136% - 144%
      Serum 143.6 140% - 147%
      Plasma 109.6 90% - 123%

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Horse

    • 製品の概要

      Horse IP-10 ELISA (CXCL10) kit is design for the quantitative measurement of horse IP-10 in serum, plasma and cell culture supernatants.


      This assay employs an antibody specific for horse IP-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IP-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-horse IP-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      This Kit detects horse IP-10.

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 試験プラットフォーム

      Pre-coated microplate (12 x 8 well strips)

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 1 x 96 tests
      200X HRP-Streptavidin Concentrate 1 x 200µl
      20X Wash Buffer 1 x 25ml
      5X Assay Diluent 1 x 15ml
      Anti-Horse IP-10  coated Microplate (12 x 8 wells) 1 unit
      Biotinylated Anti-Horse IP-10  Detection Antibody 2 vials
      Horse IP-10  Standard (Lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB Substrate Solution 1 x 12ml

    • 研究分野

    • 機能

      Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.

    • 配列類似性

      Belongs to the intercrine alpha (chemokine CxC) family.

    • 翻訳後修飾

      CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 別名

      • Interferon gamma induced factor MOB1, mouse, homolog of
      • Interferon gamma induced protein 10
      • 10 kDa interferon gamma induced protein
      • 10 kDa interferon gamma-induced protein
      • C X C motif chemokine 10
      • C7
      • Chemokine (C X C motif) ligand 10
      • Chemokine CXC motif ligand 10
      • Crg 2
      • CRG2
      • CXCL10
      • CXCL10(1-73)
      • CXL10_HUMAN
      • Gamma IP10
      • Gamma-IP10
      • gIP 10
      • GIP10
      • IFI10
      • INP 10
      • INP10
      • Interferon activated gene 10
      • Interferon gamma induced cell line
      • Interferon inducible cytokine IP 10
      • Interferon inducible cytokine IP10
      • IP 10
      • IP-10
      • Mob 1
      • MOB1
      • Protein 10 from interferon (gamma) induced cell line
      • SCYB10
      • Small inducible cytokine B10
      • Small inducible cytokine B10 precursor
      • Small inducible cytokine subfamily B (Cys X Cys) member 10
      • Small inducible cytokine subfamily B CXC member 10
      • Small inducible cytokine subfamily B, member 10
      • Small-inducible cytokine B10
      see all

    • 参照データベース

      関連製品

        アプリケーション

        The Abpromise guarantee

        Our Abpromise guarantee covers the use of ab272036 in the following tested applications.

        The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

        アプリケーション Abreviews 特記事項
        Sandwich ELISA
        Use at an assay dependent concentration.
        特記事項
        Sandwich ELISA
        Use at an assay dependent concentration.

        画像

        • Horse IP-10 standard curve
          Horse IP-10 standard curve

          This standard curve is for demonstration only. A standard curve must be run with each assay.

          Equine IP-10 ELISA kit (ab272036) Standard curve.

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