Horse IL-2 ELISA kit (ab272027)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.22 ng/ml - 160 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse
製品の概要
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製品名
Horse IL-2 ELISA kit
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.2 ng/ml -
検出範囲
0.22 ng/ml - 160 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 81.71 69% - 96% Serum 83.62 68% - 103% Plasma 79.81 69% - 92% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Horse -
製品の概要
Horse IL-2 ELISA kit is designed for the quantitative determination of Equine IL-2 in cell culture supernatants, plasma and serum samples.
ΔNote: (Equine IL-2 concentration is low in normal serum/plasma and may not be detectable in this assay
This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Equine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This kit detects equine IL-2.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Horse IL-2 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Horse IL-2 Detection Antibody 2 vials Horse IL-2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Equine IL-2 Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
Equine IL-2 ELISA kit (ab272027) Standard curve.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272027 は論文での使用が確認できていません。