Anti-Hormone sensitive lipase/HSL (phospho S853) 抗体 [EPR2329(2)] (ab109400)
![Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)](https://www.abcam.co.jp/ps/products/109/ab109400/Images/ab109400-122207-anti-hormone-sensitive-lipase-phospho-s853-antibody-epr23292-western-blot.jpg "Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)]
Hormone sensitive lipase/HSL 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853) -
由来種
Rabbit -
特異性
The antibody only detects HSL phosphorylated on serine 853. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Human skeletal muscle lysates and Mouse muscle lysates
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2329(2) -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa).
|
| 特記事項 |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa). |
追加情報
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
ターゲット情報
-
機能
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
パスウェイ
Glycerolipid metabolism; triacylglycerol degradation. -
配列類似性
Belongs to the 'GDXG' lipolytic enzyme family. -
細胞内局在
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
-
参照データベース
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Entrez Gene: 25330 Rat
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- SwissProt: P15304 Rat
see all -
別名
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
画像
-
Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)](https://www.abcam.co.jp/ps/products/109/ab109400/Images/ab109400-122206-anti-hormone-sensitive-lipase-phospho-s853-antibody-epr23292-western-blot.jpg)
Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted? -
![Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)](https://www.abcam.co.jp/ps/products/109/ab109400/Images/ab109400-3-benefits-of-recombinant-antibodies.png)
Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab109400 は 2 報の論文で使用されています。
- Wu W et al. MicroRNA-29b/29c targeting CTRP6 influences porcine adipogenesis via the AKT/PKA/MAPK Signalling pathway. Adipocyte 10:264-274 (2021). PubMed: 33938394
- Feng J et al. IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. Cell Mol Immunol 15:493-505 (2018). PubMed: 28194019
