Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (intestine)
Permeabilization
Yes - Reveal Decloaker RTU
Specification
intestine
Blocking step
Dako protein block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: A21428 - Invitrogen
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Antibody did not work well for us.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
John Freund
Verified customer
投稿 Nov 28 2018