Anti-Homer1 抗体 [EPR23178-35] - BSA and Azide free (ab269952)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23178-35] to Homer1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-Homer1 antibody [EPR23178-35] - BSA and Azide free
Homer1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23178-35] to Homer1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF,IHC-Fr or IHC-P -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse brain, hippocampus and lung lysates; Rat brain and hippocampus lysates. IP: Mouse b rain lysate.
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特記事項
ab269952 is the carrier-free version of ab245231.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23178-35 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269952の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. -
配列類似性
Belongs to the Homer family.
Contains 1 WH1 domain. -
ドメイン
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. -
細胞内局在
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 26556 Mouse
- Entrez Gene: 29546 Rat
- SwissProt: Q9Z2Y3 Mouse
- SwissProt: Q9Z214 Rat
- Unigene: 37533 Mouse
- Unigene: 37500 Rat
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別名
- HOME1_HUMAN antibody
- Homer antibody
- HOMER 1A antibody
see all
画像
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Homer1 was immunoprecipitated from 0.35 mg Mouse brain lysate with ab245231 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab245231 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain lysate 10 μg
Lane 2: ab245231 IP in Mouse brain lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245231 in Mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245231).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab269952 は論文での使用が確認できていません。