Anti-HNRPDL 抗体 (ab272661)
Key features and details
- Rabbit polyclonal to HNRPDL
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HNRPDL antibody -
製品の詳細
Rabbit polyclonal to HNRPDL -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human HNRPDL aa 100-200.
Database link: O14979 -
ポジティブ･コントロール
- WB: RT4, U-251 MG cells, human liver and tonsil tissue. IHC-P: Human cerebral tissue. IF: U-251 MG cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence. -
組織特異性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells. -
配列類似性
Contains 2 RRM (RNA recognition motif) domains. -
翻訳後修飾
Dimethylation of Arg-408 is probably of the asymmetric type. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 9987 Human
- Entrez Gene: 50926 Mouse
- Omim: 607137 Human
- SwissProt: O14979 Human
- SwissProt: Q9Z130 Mouse
- Unigene: 527105 Human
- Unigene: 707013 Human
- Unigene: 389579 Mouse
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別名
- A+U-rich element RNA binding factor antibody
- AA407431 antibody
- AA959857 antibody
see all
画像
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Western blot - Anti-HNRPDL antibody (ab272661)Lanes 2-6 : Anti-HNRPDL antibody (ab272661) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
Lane 3 : U-251 MG cells (Human brain glioma cell line)
Lane 4 : Human plasma
Lane 5 : Human liver tissue
Lane 6 : Human tonsil tissue -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPDL antibody (ab272661)Paraffin-embedded formalin-fixed human cerebral tissue stained for HNRPDL using ab272661 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HNRPDL antibody (ab272661)PFA fixed, Triton X-100 permeablized U-251 MG cells (human brain glioma cell line) labeling HNRPDL (green) using ab272661 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272661 は論文での使用が確認できていません。