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  1. Link

    hnrnp-l-antibody-4d11-ab6106.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nucleoplasm
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Anti-hnRNP L 抗体 [4D11] (ab6106)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (36)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-hnRNP L antibody [4D11] (ab6106)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)
  • Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)
  • Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)

Key features and details

  • Mouse monoclonal [4D11] to hnRNP L
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human, Newt
  • Isotype: IgG1

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-hnRNP L antibody [4D11]
    hnRNP L 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [4D11] to hnRNP L
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, WB, IP, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Newt
  • 免疫原

    Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    Purified from supernatant.
  • ポリ/モノ

    モノクローナル
  • クローン名

    4D11
  • ミエローマ

    Sp2/0
  • アイソタイプ

    IgG1
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

関連製品

  • Alternative Versions

    • Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    • Anti-ATP5D antibody [4D11] (ab174438)
    • HRP Anti-6X His tag® antibody [4D11] (ab202004)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • FITC Anti-LLGL1 antibody [4D11] (ab95802)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

アプリケーション

Our Abpromise guarantee covers the use of ab6106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • 配列類似性

    Contains 3 RRM (RNA recognition motif) domains.
  • 翻訳後修飾

    Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • 細胞内局在

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P14866 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3191 Human
    • Entrez Gene: 15388 Mouse
    • Entrez Gene: 80846 Rat
    • Omim: 603083 Human
    • SwissProt: P14866 Human
    • SwissProt: Q8R081 Mouse
    • Unigene: 446623 Human
    • Unigene: 644906 Human
    • Unigene: 9043 Mouse
    • Unigene: 33873 Rat
    see all
  • 別名

    • D830027H13Rik antibody
    • FLJ35509 antibody
    • Heterogeneous nuclear ribonucleoprotein L antibody
    • hnRNP L antibody
    • hnRNP-L antibody
    • Hnrnpl antibody
    • hnRPL antibody
    • HNRPL_HUMAN antibody
    • P/OKcl.14 antibody
    see all

画像

  • Western blot - Anti-hnRNP L antibody [4D11] (ab6106)
    Western blot - Anti-hnRNP L antibody [4D11] (ab6106)
    All lanes : Anti-hnRNP L antibody [4D11] (ab6106) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa
    Additional bands at: 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)
    IHC image of ab6106 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6106, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)
    Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)
    hnRNP L was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP L (ab6106) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6106.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 65kDa: hnRNP L. Non specific - 70kDa and 55kDa: We are unsure as to the identity of this extra band.
  • Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)
    Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)
    Overlay histogram showing Jurkat cells stained with ab6106 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6106, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

プロトコール

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (36)

    ab6106 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6106 は 36 報の論文で使用されています。

    • Van Nostrand EL  et al. Principles of RNA processing from analysis of enhanced CLIP maps for 150 RNA binding proteins. Genome Biol 21:90 (2020). PubMed: 32252787
    • Mallory MJ  et al. Reciprocal regulation of hnRNP C and CELF2 through translation and transcription tunes splicing activity in T cells. Nucleic Acids Res 48:5710-5719 (2020). PubMed: 32338744
    • Zhao L  et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon ß1 and ISG Expression. Front Microbiol 11:260 (2020). PubMed: 32153544
    • Ji J  et al. Long Noncoding RNA SChLAP1 Forms a Growth-Promoting Complex with HNRNPL in Human Glioblastoma through Stabilization of ACTN4 and Activation of NF-?B Signaling. Clin Cancer Res 25:6868-6881 (2019). PubMed: 31492748
    • Majewski L  et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018). PubMed: 29293066
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-hnRNP L antibody [4D11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (B-cell lymphocyte whole cell lysate)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Protein G
    Specification
    B-cell lymphocyte whole cell lysate
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 13 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP L antibody [4D11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblast)
    Permeabilization
    Yes - 0.3% Triton X-100 in blocking buffer
    Specification
    fibroblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 11 2018

    Western blot abreview for Anti-hnRNP L antibody [4D11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 25 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP L antibody [4D11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (human corneal endothelial cells in vitro)
    Specification
    human corneal endothelial cells in vitro
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 1% Triton
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Read More

    Dr. Zhiguo He

    Verified customer

    投稿 Nov 06 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-hnRNP L antibody [4D11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (corneal epithelium)
    Specification
    corneal epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 1% Triton
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°°C
    Read More

    Dr. Zhiguo He

    Verified customer

    投稿 Nov 05 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP L antibody [4D11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (corneal endothelium)
    Specification
    corneal endothelium
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 1% Triton
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Read More

    Dr. Zhiguo He

    Verified customer

    投稿 Nov 05 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP L antibody [4D11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Lymphoblastoid cell line)
    Specification
    Lymphoblastoid cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 13 2007

    Western blot abreview for Anti-hnRNP L antibody [4D11]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - other (rat oligodendrocytes nuclear or cytoplasmic)
    Specification
    rat oligodendrocytes nuclear or cytoplasmic
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 28 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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