Anti-hnRNP K 抗体 (ab18195)
Key features and details
- Rabbit polyclonal to hnRNP K
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-hnRNP K antibody
hnRNP K 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP K -
由来種
Rabbit -
特異性
This antibody strongly detects a single band at ~65 kDa. The predicted molecular weight of hnRNP K is ~51 kDa, but it has been shown to migrate around 65 kDa (see Milak and Clements, Nucleic Acids Research, vol 32(18), pp 5553-5569). This band is competed away by the addition of the immunizing peptide. -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Chicken, Zebrafish
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- ab18195 gave a positive result in Mouse Testis and Ovary Tissue Lysates, HeLa nuclear extract and the following whole cell lysates: HeLa; Jurkat; A431; 293T; NIH3T3; MEF1; PC12. This antibody gave a positive result in IHC in the following FFPE tissue: Human Colon Adenocarcinoma.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18195の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
-
機能
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
配列類似性
Contains 3 KH domains. -
翻訳後修飾
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
-
参照データベース
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 100008849 Rabbit
- Entrez Gene: 117282 Rat
- Entrez Gene: 406267 Zebrafish
- Omim: 600712 Human
- SwissProt: Q5ZIQ3 Chicken
- SwissProt: P61978 Human
see all -
別名
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
画像
-
Western blot - Anti-hnRNP K antibody (ab18195)All lanes : Anti-hnRNP K antibody (ab18195) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line)
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : Ovary (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody (ab18195)IHC image of hnRNP K staining in Human Colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18195, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody (ab18195)ICC/IF image of ab18195 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18195, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Panel A (left) shows localisation of the antibody (green) to specific foci in the nucleus, panel B (right) does not show the Alexa Fluor® 488 channel for comparison.
-
Western blot - Anti-hnRNP K antibody (ab18195) -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody (ab18195)ab18195 staining hnRNP K in HeLa cells treated with PD 98059 (ab120234), by ICC/IF. Changes in localization of hnRNP K (translocation from cytoplasm to nucleous) correlates with increased concentration of hnRNP K, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120234 ( hnRNP K) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18195(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab18195 は 9 報の論文で使用されています。
- Hoff FW et al. Proteomic Profiling of Acute Promyelocytic Leukemia Identifies Two Protein Signatures Associated with Relapse. Proteomics Clin Appl 13:e1800133 (2019). PubMed: 30650251
- Zhou F et al. Pim1 Impacts Enterovirus A71 Replication and Represents a Potential Target in Antiviral Therapy. iScience 19:715-727 (2019). PubMed: 31476618
- Mittenberg AG et al. Combined treatment of human multiple myeloma cells with bortezomib and doxorubicin alters the interactome of 20S proteasomes. Cell Cycle 17:1745-1756 (2018). PubMed: 30009671
- Russo J et al. Sequences encoding C2H2 zinc fingers inhibit polyadenylation and mRNA export in human cells. Sci Rep 8:16995 (2018). PubMed: 30451889
- Moujalled D et al. Kinase Inhibitor Screening Identifies Cyclin-Dependent Kinases and Glycogen Synthase Kinase 3 as Potential Modulators of TDP-43 Cytosolic Accumulation during Cell Stress. PLoS One 8:e67433 (2013). ICC/IF ; Human . PubMed: 23840699
- van Domselaar R et al. Granzyme M targets host cell hnRNP K that is essential for human cytomegalovirus replication. Cell Death Differ : (2012). PubMed: 23099853
- Pihlajamäki J et al. Expression of the splicing factor gene SFRS10 is reduced in human obesity and contributes to enhanced lipogenesis. Cell Metab 14:208-18 (2011). PubMed: 21803291
- Bovenschen N et al. Granzyme K displays highly restricted substrate specificity that only partially overlaps with granzyme A. J Biol Chem 284:3504-12 (2009). WB ; Human . PubMed: 19059912
- Rahman-Roblick R et al. p53 targets identified by protein expression profiling. Proc Natl Acad Sci U S A 104:5401-6 (2007). WB ; Human . PubMed: 17372198